Macromolecular metabolosome formation associated with 12-propanediol utilisation

与 12-丙二醇利用相关的大分子代谢体形成

• 批准号: BB/E010563/1
• 负责人: Martin Warren
• 金额: $ 81.57万
• 依托单位: University of Kent
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FE010563%2F1
• 关键词: Macromolecular; metabolosome; formation; associated; 12

Propanediol is produced as a result of the fermentation of several plant sugars and it is found in quite high concentrations in the intestine. A number of enteric bacteria, such as Salmonella enterica, have the ability to utlise propanediol as a carbon source. However, what is remarkable about this metabolism is that these bacteria actually make their own proteinaceous organelle, or microcompartment, to house this metabolic activity. The proteinaceous organelle is called a metabolsome and its formation is associated with about twenty proteins, some of which form the shell of the capsid, some of which are associated with the metabolic enzymes and some of which are associated with transport, scaffolding and regulation. The genes for propanediol utilisation are contiguous and coregulated with the cobalamin biosynthetic genes, indicating that propanediol catabolism is the primary reason for de novo B12 synthesis in many bacteria. In this application we wish to study the form and function of the propanediol metabolosome by initiating research into the organelle at the molecular level. The aim of this application is to investigate the propanediol metabolosome. We plan to study the role played by the twenty or so proteins in the metabolic process, including their function and localisation. Moreover, we will investigate the metabolic advantage of the metabolosome over the production of individual enzymes. Finally, we will explore structural aspects of the metabolosome including the ability to transport elements into and out of the macromolecular complex

丙二醇是由几种植物糖发酵产生的，在肠道中的浓度相当高。许多肠道细菌，例如肠道沙门氏菌，能够利用丙二醇作为碳源。然而，这种新陈代谢的显着之处在于，这些细菌实际上制造了自己的蛋白质细胞器或微室来容纳这种代谢活动。蛋白质细胞器称为代谢体，其形成与大约二十种蛋白质相关，其中一些蛋白质形成衣壳的外壳，一些与代谢酶相关，一些与运输、支架和调节相关。丙二醇利用基因与钴胺素生物合成基因相邻并共同调控，表明丙二醇分解代谢是许多细菌中从头合成 B12 的主要原因。在此应用中，我们希望通过在分子水平上启动对细胞器的研究来研究丙二醇代谢体的形式和功能。本应用的目的是研究丙二醇代谢体。我们计划研究二十种左右的蛋白质在代谢过程中所发挥的作用，包括它们的功能和定位。此外，我们将研究代谢体相对于单个酶的产生的代谢优势。最后，我们将探讨代谢体的结构方面，包括将元素转运入和转运出大分子复合物的能力

项目成果

Characterisation of PduS, the pdu metabolosome corrin reductase, and evidence of substructural organisation within the bacterial microcompartment.

• DOI: 10.1371/journal.pone.0014009
• 发表时间: 2010-11-16
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Parsons JB;Lawrence AD;McLean KJ;Munro AW;Rigby SE;Warren MJ
• 通讯作者: Warren MJ