OXIDATIVE STRESS AND PERFUSION IN SEPSIS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。严重脓毒症是对感染的一种炎症反应，与氧化应激和微循环改变有关。过氧化脂质膜是氧化应激的重要产物，干扰细胞的正常功能，可导致细胞死亡。虽然在人类脓毒症中已经描述了脂质过氧化，但关于败血症中脂质过氧化与血管运动功能障碍之间的关系还知之甚少。这些研究人员假设，氧化应激引起的脂质过氧化导致进行性内皮细胞损伤，不可逆转的血管运动功能障碍，以及难以治疗脓毒症的微血管灌流受损。血管舒缩功能的传统测量方法需要专门的设备和人员，因此很难用于研究，例如脓毒症的研究，当受试者无法安排时间时。这些研究人员设计了一项先导性研究，以获得测量微血管血流灌注的新的非侵入性技术的经验。这些技术包括近红外光谱(NIRS)和侧流暗场(SDF)成像。他们将把这些测量与脂质过氧化、sE-选择素以及器官功能障碍的测量进行比较。这些技术随后可以应用于正式研究，旨在调查脓毒症中氧化应激、微血管功能障碍和器官损伤之间的关系。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Severe sepsis is an inflammatory response to infection that is associated with oxidative stress and microcirculatory changes. Peroxidized lipid membranes are an important product of oxidative stress, interfere with normal cell function, and can lead to cell death. Although lipid peroxidation has been described in human sepsis, little is known regarding the relationships between lipid peroxidation and vasomotor dysfunction in sepsis. These investigators hypothesize that lipid peroxidation from oxidative stress leads to progressive endothelial cell injury, irreversible vasomotor dysfunction, and impaired microvascular perfusion that is refractory to therapy sepsis. Traditional measures of vasomotor function require specialized equipment and personnel and are therefore difficult to employ in studies, such as those of sepsis, when subjects cannot be scheduled. These investigators have designed a pilot study to gain experience with new non-invasive techniques of measuring microvascular perfusion. These techniques include Near InfraRed Spectrometry (NIRS) and Sidestream DarkField (SDF) imaging. They will compare these measurements to measures of lipid peroxidation, sE-selectin, as well as organ dysfunction. These techniques can then be applied to formal studies designed to investigate the relationships between oxidative stress, microvascular dysfunction, and organ injury in sepsis.