OXIDATIVE STRESS AND PERFUSION IN SEPSIS

脓毒症中的氧化应激和灌注

• 批准号: 7377076
• 负责人: KEVIN C DOERSCHUG
• 金额: $ 0.5万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7377076
• 关键词: OXIDATIVE; STRESS; PERFUSION; SEPSIS

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Severe sepsis is an inflammatory response to infection that is associated with oxidative stress and microcirculatory changes. Peroxidized lipid membranes are an important product of oxidative stress, interfere with normal cell function, and can lead to cell death. Although lipid peroxidation has been described in human sepsis, little is known regarding the relationships between lipid peroxidation and vasomotor dysfunction in sepsis. These investigators hypothesize that lipid peroxidation from oxidative stress leads to progressive endothelial cell injury, irreversible vasomotor dysfunction, and impaired microvascular perfusion that is refractory to therapy sepsis. Traditional measures of vasomotor function require specialized equipment and personnel and are therefore difficult to employ in studies, such as those of sepsis, when subjects cannot be scheduled. These investigators have designed a pilot study to gain experience with new non-invasive techniques of measuring microvascular perfusion. These techniques include Near InfraRed Spectrometry (NIRS) and Sidestream DarkField (SDF) imaging. They will compare these measurements to measures of lipid peroxidation, sE-selectin, as well as organ dysfunction. These techniques can then be applied to formal studies designed to investigate the relationships between oxidative stress, microvascular dysfunction, and organ injury in sepsis.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。严重脓毒症是对感染的炎症反应，与氧化应激和微循环变化相关。过氧化脂质膜是氧化应激的重要产物，干扰正常细胞功能，并可导致细胞死亡。虽然脂质过氧化反应已被描述在人类脓毒症，很少有人知道关于脂质过氧化反应和脓毒症的血管功能障碍之间的关系。这些研究者假设，氧化应激引起的脂质过氧化导致进行性内皮细胞损伤、不可逆的血管功能障碍和微血管灌注受损，这对脓毒症治疗是难治性的。血管功能的传统测量需要专门的设备和人员，因此难以在研究中使用，例如败血症研究，当受试者无法安排时。这些研究人员设计了一项试点研究，以获得新的非侵入性技术测量微血管灌注的经验。这些技术包括近红外光谱（NIRS）和侧流暗场（SDF）成像。他们将这些测量结果与脂质过氧化、sE-选择素以及器官功能障碍的测量结果进行比较。这些技术可以应用于正式的研究，旨在探讨氧化应激，微血管功能障碍，脓毒症和器官损伤之间的关系。