ROLE OF LEUKOTRIENE B4 METABOLISM IN SEVERE ASTHMA

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。本研究探讨了细胞色素P4F3在临床哮喘中的潜在作用。40名严重或持续性哮喘患者将从哮喘和阻塞性疾病诊所招募，而40名对照将从一般社区招募。将抽取血液进行白三烯代谢的遗传学和评估，呼气冷凝液将被收集用于评估NO和白三烯水平，以及IgE和RAST测试。此外，对照受试者将完成一份问卷并接受肺活量测试。患者将接受4-6个月的临床、教育、生理和药理学评估和优化，包括大剂量吸入皮质酮和长效吸入β-激动剂，以减轻症状和优化肺功能，之后他们将再次前往GCRC进行血液和呼气冷凝物测试。在门诊GCRC就诊期间，护理人员将被要求填写问卷，获得知情同意，并抽取和处理血液样本。还要求提供生物统计学支持，以协助样本能力的分析。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This study examines the potential contribution of CYP4F3 to clinical asthma. 40 patients with severe or persistent asthma will be recruited from the Asthma and Obstructive Disease clinics, while 40 controls will be recruited from the community at large. Blood will be drawn for genetics and evaluation of leukotriene metabolism, and the exhaled breath condensate will be collected for evaluation of NO and leukotriene levels, as well as IgE and RAST testing. In addition, the control subjects will complete a questionnaire and undergo spirometry testing. The patients will receive 4-6 months of clinical, educational, physiological, and pharmacological assessment and optimization, including high doses of inhaled corticosterones and long-acting inhaled beta-agonists to minimize symptoms and optimize lung function, after which they will again visit the GCRC for blood and exhale breath condensate testing. During the outpatient GCRC visits, nursing staff will be asked to administer the questionnaire, obtain informed consent, and draw and process blood samples. Biostatistics support is also requested to assist with the analysis of sample power.