Measuring biomolecular interactions using the Biacore T100

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Key to understanding biological systems is the ability to work out how different components (e.g. proteins, DNA, small molecules) interact with each other. Huge advances in measuring such interactions have occurred in recent years with the development of a technique called surface-plasmon resonance (SPR). For the past 6 years we have successfully employed a Biacore X instrument, which is a basic SPR apparatus, to make measurements in a variety of systems. However, we have become acutely aware of the limitations of the X. Last year Biacore launched its latest development in SPR technology, the Biacore T100, which contains impressive technological advances over the X, enabling measurements of greatly increased sensitivity and sophistication. We describe research programmes in plant and microbial sciences that will benefit enormously from the acquisition of a Biacore T100 instrument.