A multiuser confocal laser scanning microscope for live and 3-D imaging of craniofacial cellular dynamics

用于颅面细胞动力学实时 3D 成像的多用户共焦激光扫描显微镜

• 批准号: BB/E01335X/1
• 负责人: Jeremy Green
• 金额: $ 30.26万
• 依托单位: King's College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FE01335X%2F1
• 关键词: multiuser; confocal; laser; scanning; microscope

Birth defects, cancer, regeneration and degeneration are all processes that can be better understood by the study of normal development of embryos. Recent advances in the field have shifted the focus from individual genes to complex regulatory cascades that operate in diverse sub-cellular compartments, between cells and between tissues. This, in turn, requires the study of the distribution of gene products (proteins) and their biological effects at a very fine scale: the scale of individual cells and even within cells. Fortunately, this is facilitated by new types of microscope. The one being requested in this application is a confocal laser scanning microscope that, by illuminating only one tiny spot at a time within a specimen (but scanning the illuminating laser beam progressively across and through it), eliminates most of the glare and fuzz of conventional microscopes. Such instruments enable images from deep within tissues to be gathered and different colours that identify different proteins to be detected simultaneously. The department requesting this instrument does not have its own confocal microscope, and several members of the department currently have to go to remote sites to collect these crucial images. The provision of the requested instrument will thus result in greatly increased efficiency in using Research Council and other funds for project work.

出生缺陷、癌症、再生和退化都是通过研究胚胎的正常发育可以更好地理解的过程。该领域的最新进展已经将焦点从单个基因转移到在细胞之间和组织之间的不同亚细胞区室中操作的复杂调控级联。这反过来又要求研究基因产物（蛋白质）的分布及其在非常精细的尺度上的生物学效应：单个细胞甚至细胞内的尺度。幸运的是，这是由新型显微镜促进的。本申请中要求的是共焦激光扫描显微镜，它通过一次仅照射样本内的一个微小斑点（但照射激光束逐渐扫描并穿过它），消除了传统显微镜的大部分眩光和模糊。这些仪器能够收集来自组织深处的图像，并同时检测识别不同蛋白质的不同颜色。要求使用该仪器的部门没有自己的共聚焦显微镜，该部门的几名成员目前不得不前往偏远地点收集这些关键图像。因此，提供所要求的工具将大大提高研究理事会和其他项目工作资金的使用效率。

项目成果

Pervasive cortical and white matter anomalies in a mouse model for CHARGE syndrome.

• DOI: 10.1111/joa.13856
• 发表时间: 2023-07
• 期刊: Journal of anatomy
• 影响因子: 2.4

Lrp4 modulates extracellular integration of cell signaling pathways in development.

• DOI: 10.1371/journal.pone.0004092
• 发表时间: 2008
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Ohazama A;Johnson EB;Ota MS;Choi HY;Porntaveetus T;Oommen S;Itoh N;Eto K;Gritli-Linde A;Herz J;Sharpe PT
• 通讯作者: Sharpe PT

The planar cell polarity effector Fuz is essential for targeted membrane trafficking, ciliogenesis and mouse embryonic development.

• DOI: 10.1038/ncb1966
• 发表时间: 2009-10
• 期刊: NATURE CELL BIOLOGY
• 影响因子: 21.3
• 作者: Gray, Ryan S.;Abitua, Philip B.;Wlodarczyk, Bogdan J.;Szabo-Rogers, Heather L.;Blanchard, Otis;Lee, Insuk;Weiss, Greg S.;Liu, Karen J.;Marcotte, Edward M.;Wallingford, John B.;Finnell, Richard H.
• 通讯作者: Finnell, Richard H.

Pax2 coordinates epithelial morphogenesis and cell fate in the inner ear.

• DOI: 10.1016/j.ydbio.2010.07.007
• 发表时间: 2010-09-15
• 期刊: Developmental biology
• 影响因子: 2.7
• 作者: Christophorou NA;Mende M;Lleras-Forero L;Grocott T;Streit A
• 通讯作者: Streit A

Epithelial stratification and placode invagination are separable functions in early morphogenesis of the molar tooth.

• DOI: 10.1242/dev.130187
• 发表时间: 2016-02-15
• 期刊: Development (Cambridge, England)
• 作者: Li J;Chatzeli L;Panousopoulou E;Tucker AS;Green JB
• 通讯作者: Green JB