Structure of Dazl based translation control complexes

基于 Dazl 的翻译控制复合体的结构

• 批准号: BB/E020070/1
• 负责人: Thomas Edwards
• 金额: $ 51.37万
• 依托单位: University of Leeds
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FE020070%2F1
• 关键词: Structure; Dazl; based; translation; control

Human fertility depends upon the production of mature sperm and eggs, and defects in the control of gene expression during this process contribute to infertility, a problem affecting 12-15% of couples worldwide. Deletions on the Y chromosome in infertile men suggested a fertility factor at this location and subsequent mapping identified the Deleted in AZoospermia (DAZ) gene. Members of the DAZ gene family are required for fertility in species across evolution. Mouse Dazl, a member of the DAZ gene family, is an RNA binding protein. Dazl recognises specific sequences on certain messenger RNAs in developing germ cells and recruits protein cofactors in order to regulate how much protein is produced from that mRNA, dictating expression of that gene. We aim to determine the three-dimensional structure of the Dazl protein bound to RNA or the protein cofactors. This will allow us to visualise the components of this system and how they interact at very high resolution, demonstrating how they normally function in controlling gene expression.

人类的生育能力取决于成熟精子和卵子的产生，在这个过程中基因表达控制的缺陷会导致不育，这是一个影响全球12-15%夫妇的问题。不育男性的Y染色体缺失提示了该位置的生育因素，随后的定位确定了无精子症（DAZ）基因。DAZ基因家族的成员是物种进化过程中生育力所必需的。小鼠Dazl是DAZ基因家族的成员，是一种RNA结合蛋白。Dazl识别发育中的生殖细胞中某些信使RNA上的特定序列，并招募蛋白质辅因子，以调节从该mRNA产生多少蛋白质，决定该基因的表达。我们的目标是确定结合RNA或蛋白质辅因子的Dazl蛋白的三维结构。这将使我们能够以非常高的分辨率可视化该系统的组成部分以及它们如何相互作用，从而展示它们在控制基因表达中的正常功能。

项目成果

Structure of the Drosophila melanogaster Rab6 GTPase at 1.4 Å resolution.

果蝇 Rab6 GTPase 的结构，分辨率为 1.4 ×。

• DOI: 10.1107/s1744309111017453
• 发表时间: 2011
• 期刊: Acta crystallographica. Section F, Structural biology and crystallization communications
• 作者: Walden M