Deciphering the d1 haem biosynthesis pathway and its relationship to a novel anaerobic route to protohaem

破译 d1 血红素生物合成途径及其与新的原血红素厌氧途径的关系

• 批准号: BB/E022944/1
• 负责人: Stuart John Ferguson
• 金额: $ 53.25万
• 依托单位: University of Oxford
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FE022944%2F1
• 关键词: Deciphering; d1; haem; biosynthesis; pathway

Many proteins contain haem (eg hemoglobin of the blood) which is a ring molecule with an iron atom in the middle. In some proteins the haem is modified, as is the case in an enzyme that catalyses a key reaction in the global nitrogen cycle. This reaction is reduction of nitrite to nitric oxide and the modified haem is called d1. We know a great deal about how 'standard' haem (protohaem) is made in cells from much simpler chemicals but we know almost nothing about how the vital d1 haem is made. It is our major aim to discover the steps whereby cells construct the d1 haem species. Unexpectedly, it has recently emerged that certain kinds of organisms classified as archaea do not have a recognisable haem synthesis pathway and the same is true for bacteria that reduce sulfate. The two classes of organisms do not contain nitrite reductase but do contain some predicted proteins which are very similar to those implicated in d1 haem synthesis. Thus we expect also to contribute to understanding a novel haem synthesis route with which interesting evolutionary implications are associated.

许多蛋白质都含有血红素（如血液中的血红蛋白），血红素是一种环状分子，中间有一个铁原子。在某些蛋白质中，血红素被修饰，就像催化全球氮循环中关键反应的酶一样。这个反应是将亚硝酸盐还原为一氧化氮，改性的血红素被称为d1。我们知道很多关于“标准”血红素（原血红素）是如何在细胞中从更简单的化学物质中产生的，但我们对重要的d1血红素是如何产生的几乎一无所知。我们的主要目标是发现细胞构建d1血红素种类的步骤。令人意想不到的是，最近发现某些被归类为古生菌的生物没有可识别的血红素合成途径，还原硫酸盐的细菌也是如此。这两类生物不含亚硝酸还原酶，但含有一些预测的蛋白质，这些蛋白质与d1血红素合成中涉及的蛋白质非常相似。因此，我们希望也有助于了解一种新的血红素合成途径，有趣的进化的影响。