The assembly of mitochondrial c-type cytochromes by System III

System III 组装线粒体 c 型细胞色素

• 批准号: BB/H017887/1
• 负责人: Stuart John Ferguson
• 金额: $ 47.35万
• 依托单位: University of Oxford
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2010
• 资助国家: 英国
• 起止时间: 2010 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FH017887%2F1
• 关键词: assembly; mitochondrial; type; cytochromes; System

Proteins are complex biomolecules that are essential to the structure and function of all living cells. In order to diversify their functions, proteins use additional molecules called cofactors, of which there are many types. Heme is one such cofactor; it is an organic chemical with an iron atom in the centre and has many essential functions in different cellular processes. Many proteins that contain heme undergo a process after they are made in the cell in which bonds are formed between the protein and the heme; these are called cytochromes c and are essential in respiration and in reactions that involve the transfer of electrons. In animal cells there are two c-type cytochrome molecules which are both found in the mitochondria. These cytochromes play a key role underpinning the mitochondrion's role as the powerhouse of the cell. Additionally, one of these cytochromes c can be released from the mitochondrion under certain conditions as a trigger for the process of programmed cell death. In other cases, heme associates with proteins without forming bonds with them, for example in hemoglobin, which uses the heme to bind oxygen in blood. In the case where the heme cofactor becomes bonded to the cytochrome, other proteins are needed to assist in this reaction, which is not spontaneous. In animals a single protein speeds up the covalent attachment of heme to protein to give a cytochrome c. Our proposed research seeks to understand how this protein functions, using a variety of experimental approaches and techniques. It is also important to understand the process of making cytochromes c as a fundamental and central biological process, in order to complete a description of the essential biochemical reactions that constitute life.

蛋白质是复杂的生物分子，对所有活细胞的结构和功能至关重要。为了使其功能多样化，蛋白质使用称为辅因子的额外分子，其中有许多类型。血红素就是这样一种辅因子;它是一种中心有一个铁原子的有机化学物质，在不同的细胞过程中具有许多重要功能。许多含有血红素的蛋白质在细胞中形成后，会经历一个过程，在蛋白质和血红素之间形成键;这些被称为细胞色素c，在呼吸和涉及电子转移的反应中是必不可少的。在动物细胞中，有两种c型细胞色素分子，它们都存在于线粒体中。这些细胞色素起着关键作用，支撑着细胞色素作为细胞动力的作用。此外，这些细胞色素c中的一种可以在某些条件下作为程序性细胞死亡过程的触发剂从细胞色素释放。在其他情况下，血红素与蛋白质结合而不与它们形成键，例如在血红蛋白中，血红蛋白使用血红素结合血液中的氧气。在血红素辅因子与细胞色素结合的情况下，需要其他蛋白质来帮助这种反应，这不是自发的。在动物中，单个蛋白质加速血红素与蛋白质的共价结合，产生细胞色素c。我们提出的研究旨在使用各种实验方法和技术来了解这种蛋白质的功能。为了完整地描述构成生命的基本生化反应，理解细胞色素c的生成过程是一个基本的、中心的生物学过程也是很重要的。

项目成果

The mitochondrial cytochrome c N-terminal region is critical for maturation by holocytochrome c synthase.

线粒体细胞色素 c N 末端区域对于全细胞色素 c 合酶的成熟至关重要。

• DOI: 10.1016/j.febslet.2011.04.058
• 发表时间: 2011
• 期刊: FEBS letters
• 影响因子: 3.5
• 作者: Stevens JM

A pivotal heme-transfer reaction intermediate in cytochrome c biogenesis.

• DOI: 10.1074/jbc.m111.313692
• 发表时间: 2012-01-20
• 期刊: The Journal of biological chemistry
• 作者: Mavridou DA;Stevens JM;Mönkemeyer L;Daltrop O;di Gleria K;Kessler BM;Ferguson SJ;Allen JW
• 通讯作者: Allen JW

The interplay between the disulfide bond formation pathway and cytochrome c maturation in Escherichia coli.

• DOI: 10.1016/j.febslet.2012.04.055
• 发表时间: 2012-06-12
• 期刊: FEBS letters
• 影响因子: 3.5
• 作者: Mavridou DA;Ferguson SJ;Stevens JM
• 通讯作者: Stevens JM

Substrate recognition of holocytochrome c synthase: N-terminal region and CXXCH motif of mitochondrial cytochrome c.

• DOI: 10.1016/j.febslet.2014.07.026
• 发表时间: 2014-09-17
• 期刊: FEBS letters
• 影响因子: 3.5
• 作者: Zhang Y;Stevens JM;Ferguson SJ
• 通讯作者: Ferguson SJ

Probing heme delivery processes in cytochrome c biogenesis System I.

• DOI: 10.1021/bi400398t
• 发表时间: 2013-10-15
• 期刊: BIOCHEMISTRY
• 影响因子: 2.9
• 作者: Mavridou, Despoina A. I.;Clark, Matthew N.;Choulat, Cendie;Ferguson, Stuart J.;Stevens, Julie M.
• 通讯作者: Stevens, Julie M.