ANTICANCER AND ANTI-INFECTIVE METABILITES FOR SYMBIOTIC MARINE MICROORGANISMS

共生海洋微生物的抗癌和抗感染代谢物

• 批准号: 7381336
• 负责人: KHALID A EL SAYED
• 金额: $ 2.5万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7381336
• 关键词: ANTICANCER; ANTI; INFECTIVE; METABILITES; SYMBIOTIC

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The abundance of drug-resistant bacterial infections and cancers has generated an urgent interest in the search for prototype antibiotic and anticancer leads. Oceans nearly possess 80% of Earth's animal life. The chemical and biological diversity of marine natural products make them an enormous resource of unique and highly bioactive compounds. Studies suggest that many bioactive compounds isolated from marine invertebrates such as sponges, coelenterates, mollusks, or protochordates, are truly originating from symbiotic microorganisms, e.g., bacteria, fungi, blue green algae, dinoflagellates, or haptophytes. Marine microorganisms are the most diverse and renewable resources of new compounds, which are very likely to have biological activity including anticancer or antiinfective effects. Most of marine microorganisms live symbiotically in other soft-bodied marine organisms, which lack obvious structural defenses. This study will explore the ability of cultured microorganisms to produce latrunculins, sipholanes, cembranoids, and any related anticancer/anti-infective secondary metabolites. Hypothesis of this proposal is: bioactive natural products are produced by symbiotic microorganisms associated with the Red Sea sponge N. magnifica, and S. siphonella, and the soft coral S. glaucum. These compounds can be produced on large scale by in vitro culturing of their source microorganisms. This hypothesis will be tested by pursuing the following specific aims: 1- Isolation/identification of symbiotic microorganisms associated with the aforementioned animals. 2- Large-scale culturing of the isolated microorganism and testing their extracts for latrunculins, sipholanes, cembranoids, and other novel secondary metabolites. 3- Evaluation of anticancer and antibiotic activity of the extracts and isolated compounds.

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。大量的耐药细菌感染和癌症引起了人们对寻找原型抗生素和抗癌线索的迫切兴趣。海洋几乎拥有地球上80%的动物。海洋天然产物的化学和生物多样性使它们成为独特和高度生物活性化合物的巨大资源。研究表明，从海绵、腔肠动物、软体动物或原脊索动物等海洋无脊椎动物中分离出的许多生物活性化合物，实际上是源自共生微生物，如细菌、真菌、蓝绿藻、鞭毛藻或触媒生物。海洋微生物是新化合物最多样化和可再生的资源，这些新化合物极有可能具有抗癌或抗感染等生物活性。大多数海洋微生物与其他海洋软体生物共生，缺乏明显的结构防御。本研究将探索培养的微生物产生拉特库林、虹膜烷、类膜膜和任何相关的抗癌/抗感染次级代谢物的能力。该提案的假设是：具有生物活性的天然产物是由与红海海绵N. magnifica、S. siphonella和软珊瑚S. glaucum相关的共生微生物产生的。这些化合物可以通过其源微生物的体外培养大规模生产。这一假设将通过追求以下具体目标来验证：1-分离/鉴定与上述动物相关的共生微生物。2-对分离的微生物进行大规模培养，并对其提取物进行检测，以检测其对拉特库林、虹膜烷、类膜膜和其他新的次生代谢物的提取。3-评价提取物和分离化合物的抗癌和抗生素活性。