INBRE RESEARCH, STUDENT MENTORING AND OUTREACH PROJECT

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。LCC的外展核心研究有三个组成部分，1）学习从拟南芥中分离和表征蛋白质二硫键异构酶（PDI）基因的研究技术，作为导师，UH Manoa热带农业和人力资源学院的大卫克里斯托弗博士; 2）指导LCC学生的研究方法和3）在现代生命科学教育中对K-12学生进行外展活动。 2005年，在国家建筑研究所的支助下开展了三项外联活动。首先，两个坎贝尔高中学生黛西M。拉莫斯和埃里卡贝利K.铃木，在2004年秋季为他们的高中科学博览会项目工作。学生们使用真实的时间PCR来调查UH种子实验室的非转基因番木瓜种子批次与转基因番木瓜种子的潜在污染。埃里卡和黛西都在不同的实验室工作，并与该领域的许多科学家进行了交流，包括理查德·曼沙特教授。他们的项目题为通过使用真实的时间PCR标准确定非转基因番木瓜的污染水平？在2005年4月4日至6日举行的夏威夷州科学与工程博览会上获得以下奖项：美国化学学会夏威夷分会奖，第二名（50美元）夏威夷农业研究中心奖（50美元和证书）伽马西格玛德尔塔，农业荣誉协会奖（100美元和证书）夏威夷兽医协会奖（100美元）第二项活动涉及展示水果DNA和建造一个3-在Hokulani小学给20个金格尔花园的学生做DNA模型。 最后一次外展活动是由来自荣誉生物学班的八名学生和他们的老师从Mililani高中（MHS）到LCC进行实地考察。学生们参加了为期两天的实验室体验，在其中学生们了解了DNA是如何用来帮助解决犯罪的法医实验室。除了实验室安全，学生们还学习了几种分子技术，包括质粒分离，限制性酶切消化，琼脂糖凝胶电泳和结果的照片记录。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. LCC's outreach core research has three components, 1) learn research techniques in isolation and characterization of Protein Disulfide Isomerase (PDI) genes from Arabidopsis with Dr. David Christopher of the College of Tropical Agriculture and Human Resources, UH Manoa, as mentor; 2) mentor LCC students in research methodology and 3) conduct outreach activities to K-12 students in modern life sciences education. Three outreach activities have been performed in 2005 with INBRE support. First, two Campbell High School students Daisy M. Ramos and Erica Bailey K. Suzuki, worked for their high school science fair project in Fall 2004. The students used Real Time PCR to investigate potential contamination of non-transgenic papaya seed lots in UH seed lab with transgenic papaya seeds. Both Erica and Daisy worked in different labs and interacted with a number of scientists in the field, including Prof. Richard Manshardt. Their project entitled Determining Levels of Contamination in Non-Transgenic Carica papaya through the Use of Real Time PCR-Made Standard? received the following award in the Hawaii State Science and Engineering Fair held in April 4-6, 2005: American Chemical Society, Hawai`i Section Award, 2nd Place ($50) Hawai`i Agriculture research Center Award ($50 and certificate) Gamma Sigma Delta, the Honor Society of Agriculture Award ($100 and certificate) Hawaii Veterinary Medical Association Award ($100) The second activity involved demonstration of DNA from fruits and construction of a 3-D DNA model to 20 kingergarten students in Hokulani elementary School. The last outreach activity involved a field visit by eight students from the Honors Biology class and their teacher from Mililani High School (MHS) to LCC. Students participated in a two-day laboratory experience in which the students learned about how DNA is used to help solve crime in forensic laboratories. In addition to laboratory safety, students learned several molecular techniques, including plasmid isolation, restriction digestion, agarose gel electrophoresis and photodocumentation of results.