PUI RESEARCH-TOUGALOO-MAO

PUI 研究-TOUGALOO-MAO

• 批准号: 7381621
• 负责人: JINGHE MAO
• 金额: $ 18.17万
• 依托单位: UNIVERSITY OF SOUTHERN MISSISSIPPI
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7381621
• 关键词: PUI; RESEARCH; TOUGALOO; MAO

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In the budding yeast Saccharomyces cerevisiae, large-scale gene deletion analysis has shown that over 80% of the ~6200 yeast genes are non-essential, implying that many genes and pathways in eukaryotic cells must be functionally redundant or buffered from phenotypic consequences following genetic perturbation. I am interested in exploring this functional redundancy, especially as it relates to transcriptional regulatory networks. A number of uncharacterized open reading frames (ORFs) have sequence homology to well known families of transcription factors. Using high-throughput genetic analysis (Synthetic Genetic Array Analysis, or SGA), DNA microarrays, and Genome-wide location analysis or Chip on chip, I would like to connect one or more of these uncharacterized putative regulatory genes with a new or existing physiologically significant pathway.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。在芽殖酵母酿酒酵母中，大规模的基因缺失分析表明，约6200个酵母基因中超过80%是非必需的，这意味着真核细胞中的许多基因和途径必须是功能冗余的，或者在遗传扰动后缓冲表型后果。我有兴趣探索这种功能冗余，特别是因为它涉及到转录调控网络。许多未表征的开放阅读框（ORF）与众所周知的转录因子家族具有序列同源性。使用高通量遗传分析（合成遗传阵列分析或SGA），DNA微阵列和全基因组定位分析或芯片上芯片，我想将这些未表征的推定调控基因中的一个或多个与新的或现有的生理学重要途径联系起来。