PUI RESEARCH-TOUGALOO-HENNINGTON

PUI 研究-图加卢-亨宁顿

• 批准号: 8360564
• 负责人: BETTYE S HENNINGTON
• 金额: $ 10.59万
• 依托单位: UNIVERSITY OF SOUTHERN MISSISSIPPI
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360564
• 关键词: Cell Cycle; Cells; Complex; Eukaryota; Event; Funding; Generations; Genes; Genetic Transcription; Grant; Label; Maps; Mass Spectrum Analysis; Mississippi; Modeling; National Center for Research Resources; Phosphoproteins; Phosphorylation Site; Principal Investigator; Process; Proteins; Research; Research Infrastructure; Resources; Ribosomal Proteins; Saccharomyces cerevisiae; Signal Transduction; Source; United States National Institutes of Health; cost; functional genomics; in vivo; rapid growth

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In the model eukaryote Saccharomyces cerevisiae, cell cycle events are coordinated with constitutive functions like energy generation and duplication of protein mass. The latter processes are stimulated by the Rap1p/Gcr1p/Gcr2p complex, which activates transcription of the genes required for rapid growth; this includes glycolytic and ribosomal protein genes, which are among the most heavily transcribed in the cell. In vivo 32P labeling has shown that Rap1p, Gcr1p, and Gcr2p are all phosphoproteins. Mapping the exact sites of phosphorylation in these proteins would represent an important advance in our understanding of the signaling cascade(s) that regulates the Rap1 activation complex. Mass spectrometry (LC-MS/MS) will be used to analyze phosphorylation sites in these factors.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其他NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 在模型真核生物酿酒酵母中，细胞周期事件与组成性功能如能量产生和蛋白质质量复制相协调。后一个过程由Rap 1 p/Gcr 1 p/Gcr 2 p复合物刺激，该复合物激活快速生长所需的基因的转录;这包括糖酵解和核糖体蛋白基因，它们是细胞中转录最多的基因之一。体内~（32）P标记表明，Rap 1 p、Gcr 1 p和Gcr 2 p都是磷蛋白。绘制这些蛋白质中磷酸化的确切位点将代表我们对调节Rap 1激活复合物的信号级联的理解的重要进展。将使用质谱法（LC-MS/MS）分析这些因子中的磷酸化位点。