INBRE COLLABORATION

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。在本预算年度，与路易斯维尔大学健康科学中心的Abdelnaby Khalyfa博士和奈杰尔库珀博士发起了一个合作项目。本项目利用微阵列（小鼠全基因组）分析感染寄生虫病原体弓形虫的小鼠脑中的基因表达。通过这种分析，将有可能确定在小鼠脑组织中表达的mRNA的相对丰度，并且将有可能确定哪些基因在T.刚地氏菌感染的小鼠维持缺乏维生素E和硒的饮食，而那些维持含有高水平的这些抗氧化剂的饮食。虽然我们实验室的主要重点是免疫相关基因，但通过微阵列分析，可能会发现其他受饮食影响的基因。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During this budget year, a collaborative project was initiated with Dr. Abdelnaby Khalyfa and Dr. Nigel Cooper of the University of Louisville Health Sciences Center. This project utilizes Microarray (whole mouse genome) to analyze gene expression in the brains of mice infected with the parasitic pathogen, Toxoplasma gondii. With this analysis, it will be possible to determine the relative abundance of mRNAs expressed in mouse brain tissue, and it will be possible to determine which genes are differentially expressed between T. gondii-infected mice maintained on a diet lacking vitamin E and selenium and those maintained on a diet containing elevated levels of these antioxidants. Although the major focus of our laboratory has been on genes of immunological relevance, with microarray analysis, other genes whose expression is influenced by diet will likely be identified.