INBRE COLLABORATION

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。在本预算年度，与路易斯维尔大学健康科学中心的Abdelnaby Khalyfa博士和Nigel Cooper博士启动了一个合作项目。该项目利用基因芯片(小鼠全基因组)来分析感染弓形虫寄生虫的小鼠大脑中的基因表达。通过这一分析，将有可能确定在小鼠脑组织中表达的mRNAs的相对丰度，并将有可能确定弓形虫感染小鼠在缺乏维生素E和硒的饮食中与那些在这些抗氧化剂水平较高的饮食中保持的弓形虫感染小鼠之间的差异表达。虽然我们实验室的主要关注点是与免疫学相关的基因，但通过微阵列分析，可能会识别出其他表达受饮食影响的基因。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During this budget year, a collaborative project was initiated with Dr. Abdelnaby Khalyfa and Dr. Nigel Cooper of the University of Louisville Health Sciences Center. This project utilizes Microarray (whole mouse genome) to analyze gene expression in the brains of mice infected with the parasitic pathogen, Toxoplasma gondii. With this analysis, it will be possible to determine the relative abundance of mRNAs expressed in mouse brain tissue, and it will be possible to determine which genes are differentially expressed between T. gondii-infected mice maintained on a diet lacking vitamin E and selenium and those maintained on a diet containing elevated levels of these antioxidants. Although the major focus of our laboratory has been on genes of immunological relevance, with microarray analysis, other genes whose expression is influenced by diet will likely be identified.