COBRE: UOK HSC: P3: BIOFILM FORMATION BY ACTINOBACILLUS ACTINOMYCETEMCOMITANS

COBRE：UOK HSC：P3：放线杆菌伴放线菌形成生物膜

• 批准号: 7382015
• 负责人: Marvin Whiteley
• 金额: $ 28.71万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7382015
• 关键词: COBRE; UOK; HSC; P3; BIOFILM

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Actinobacillus actinomycetemcomitans (Aa) is a gram-negative capnophilic bacterium implicated in early onset periodontal diseases, subcutaneous abscesses, and endocarditis. Little is known about the mechanisms of pathogenesis in Aa, other than this bacterium produces a variety of potential virulence factors including a potent leukotoxin which contributes to disease (36). One of the most striking features of many clinical isolates of Aa is the ability to form dense tightly-packed biofilms which are difficult to dissociate (11-13, 30). This tight adherence has been linked to the ability of Aa to colonize the mouths of rats and may be important for persistence in humans (55). In the current application, we propose experiments designed to understand the processes required for Aa biofilm formation. We will use the recently completed Aa genome to design genome-scale strategies for studying Aa biofilm development. Although several studies involving Aa biofilm formation have been published, virtually nothing is known about the developmental pathway for biofilm formation. Our goals are to identify processes important for attachment and biofilm formation in Aa and evaluate their impact on pathogenesis and persistence in the oral cavity.

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。放线菌（放线菌）是一种革兰氏阴性嗜热细菌，与早发性牙周病、皮下脓肿和心内膜炎有关。除了这种细菌产生多种潜在的毒力因子，包括一种导致疾病的强效白质毒素外，对Aa的发病机制知之甚少（36）。许多Aa临床分离株最显著的特征之一是能够形成致密的紧密排列的生物膜，难以分离（11- 13,30）。这种紧密的粘附性与Aa在大鼠口腔中定居的能力有关，可能对人类的持久性也很重要（55）。在目前的应用中，我们提出了旨在了解Aa生物膜形成所需过程的实验。我们将利用最近完成的Aa基因组来设计研究Aa生物膜发育的基因组尺度策略。虽然已经发表了一些涉及Aa生物膜形成的研究，但实际上对生物膜形成的发育途径一无所知。我们的目标是确定Aa中附着和生物膜形成的重要过程，并评估它们对口腔发病机制和持久性的影响。