Immune Response of Drosophila Against Spiroplasma
果蝇对螺原体的免疫反应
基本信息
- 批准号:7680793
- 负责人:
- 金额:$ 0.52万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2008
- 资助国家:美国
- 起止时间:2008-02-15 至 2010-01-31
- 项目状态:已结题
- 来源:
- 关键词:AddressAlternative SplicingBacteriaBiological ModelsCell WallCell membraneClassCommunicable DiseasesComplexDefense MechanismsDiseaseDrosophila genusDrosophila melanogasterEmployee StrikesFoundationsGene Expression ProfileGenesGeneticGenomicsGoalsHemocytesHemolymphHumanImmuneImmune responseImmune systemImmunityImmunologic MemoryInfectionInvadedInvertebratesLipoproteinsMicrobeMolecularMollicutesMycoplasmaMycoplasma pneumoniaeNatural ImmunityOrganismPeptidoglycanProteomeProteomicsPublic HealthResearchSignal PathwaySpiroplasmaStagingSystemThinkingTimeToll-like receptorsUreaplasmaVertebratesVirusWorkflyfungusgenome sequencingimprovedmembermicrobialmutantpathogenpreventresearch studyresponsetool
项目摘要
DESCRIPTION (provided by applicant): Understanding the mechanisms of immune defense against pathogens is important for preventing and treating human infectious diseases. The importance of the innate branch of the human immune response is increasingly recognized as crucial in defense against many pathogens. Innate defense mechanisms of invertebrates and vertebrates show striking similarities and are evolutionary conserved. The fruitfly Drosophila melanogaster has provided an unbiased and powerful tool for examination of innate response against many types of microbial pathogens. However, it has not yet been used to explore innate response against bacteria in the Class Mollicutes, which includes important pathogens of humans such as Mycoplasma pneumoniae. The major goal of this proposal is to develop Drosophila as a model system for studying innate immunity against mollicutes, taking advantage of naturally infecting mollicutes of Drosophila (i.e., genus Spiroplasma). In the proposed study, the hypothesis is that mollicutes that naturally infect Drosophila elicit an innate immune response in the fly, which varies depending on the phenotypic effects (from mild to severe) of the bacterium strain on the host. These innate responses are likely to be conserved across invertebrates and vertebrates; thus, this work will serve as the foundation to study whether Drosophila innate immune response can help develop treatments for mollicute-related illnesses in humans and other vertebrates. The aim of the proposed project is to identify factors involved in the interaction between Drosophila and Spiroplasma via the following Specific Aims:
Aim I. Establish whether the presence of Spiroplasma in Drosophila induces changes in the hemocyte transcriptome via hemocyte microarray experiments.
Aim II. Establish whether the presence of Spiroplasma in Drosophila induces changes in the hemolymph proteome via proteomics/peptidomics experiments.
PUBLIC HEALTH RELEVANCE The proposed research will use the fruitfly Drosophila melanogaster to understand the innate response of organisms against bacteria from the class Mollicutes. Bacteria in the class Mollicutes include important pathogens of humans such as Mycoplasma pneumoniae. By understanding how Drosophila, which lacks an adaptive immune system, interacts with Mollicutes, we may be able to understand the contribution of the human innate response to defense against Mollicutes.
描述(申请人提供):了解病原体的免疫防御机制对于预防和治疗人类传染病具有重要意义。人们越来越认识到人类免疫反应的先天分支对于防御许多病原体至关重要。无脊椎动物和脊椎动物的先天防御机制表现出惊人的相似性,并且在进化上是保守的。果蝇果蝇为检查针对多种微生物病原体的先天反应提供了一种公正且强大的工具。然而,它尚未用于探索针对软体动物纲细菌的先天反应,其中包括人类重要的病原体,例如肺炎支原体。该提案的主要目标是利用果蝇(即螺原体属)的自然感染软体,将果蝇开发为研究针对软体的先天免疫的模型系统。在拟议的研究中,假设自然感染果蝇的软体动物会在果蝇中引起先天免疫反应,该反应根据细菌菌株对宿主的表型影响(从轻微到严重)而变化。这些先天反应可能在无脊椎动物和脊椎动物中都是保守的。因此,这项工作将成为研究果蝇先天免疫反应是否有助于开发人类和其他脊椎动物软体相关疾病的治疗方法的基础。拟议项目的目的是通过以下具体目标确定果蝇和螺原体之间相互作用所涉及的因素:
目标 I. 通过血细胞微阵列实验确定果蝇中螺原体的存在是否会诱导血细胞转录组的变化。
目标二。通过蛋白质组学/肽组学实验确定果蝇中螺原体的存在是否会诱导血淋巴蛋白质组的变化。
公共卫生相关性拟议的研究将使用果蝇果蝇来了解生物体对软体动物纲细菌的先天反应。软体动物纲中的细菌包括人类的重要病原体,例如肺炎支原体。通过了解缺乏适应性免疫系统的果蝇如何与软体动物相互作用,我们也许能够了解人类先天反应对防御软体动物的贡献。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
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Mariana Mateos其他文献
Mariana Mateos的其他文献
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