Combined OCT/FLIM Biochemical and Anatomical Imaging for Early Cancer Diagnosis

结合 OCT/FLIM 生化和解剖成像进行早期癌症诊断

• 批准号: 7540319
• 负责人: Javier Antonio Jo
• 金额: $ 33.46万
• 依托单位: TEXAS ENGINEERING EXPERIMENT STATION
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-15 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7540319
• 关键词: Address; Algorithms; American Cancer Society; Biochemical; Biopsy; Cancerous; Characteristics; Cheek structure; Classification; Computer Systems Development; Computing Methodologies; Contrast Media; Data; Databases; Depth; Detection; Development; Diagnosis; Diagnostic; Early Diagnosis; Epithelial; Epithelium; Fluorescence; Fluorescence Spectroscopy; Future; Goals; Hamsters; Histopathology; Image; Image Analysis; Imaging Techniques; Imaging technology; Invasive; Lesion; Malignant - descriptor; Malignant Neoplasms; Measures; Metric; Microanatomy; Modality; Modeling; Monitor; Morbidity - disease rate; Mouth Neoplasms; Multimodal Imaging; Neoplasms; Normal tissue morphology; Operative Surgical Procedures; Optical Biopsy; Optical Coherence Tomography; Optics; Oral; Oral Diagnosis; Oral cavity; Penetration; Pharyngeal structure; Premalignant; Public Health; Quality of life; Quantitative Evaluations; Range; Reporting; Research; Resolution; Screening for Oral Cancer; Screening procedure; Sensitivity and Specificity; Signal Transduction; Staging; Standards of Weights and Measures; Survival Rate; Survivors; System; Techniques; Texture; Therapeutic; Therapeutic Intervention; Time; Tissues; Visual; Work; cancer diagnosis; carcinogenesis; design; fluorescence imaging; image processing; improved; in vivo; instrumentation; malignant mouth neoplasm; molecular imaging; mortality; neoplastic; novel; novel therapeutics; optical imaging; response; size; success; tomography; tool

DESCRIPTION (provided by applicant): Early detection of neoplastic changes in the oral cavity holds great promise for improving survival rates and the quality of life of survivors. The high spatial resolution of optical imaging could make possible the diagnosis of neoplastic lesions while they are still in the submillimeter range in size and highly treatable. Both biochemical and morphological changes accompany the transition from normal tissue to neoplastic tissue. Yet the optical techniques heretofore investigated as potential screening and diagnostic tools for cancer have largely concentrated on detecting one or the other, principally because of the fundamental lack of optical imaging modalities which readily provide both types of information. Here we propose to simultaneously collect morphological and biochemical information by combining two well developed optical imaging techniques, optical coherence tomography (OCT) and fluorescence lifetime imaging (FLIM). Since OCT and FLIM measure different tissue characteristics (structural and biochemical, respectively), we hypothesize that their synergy will increase the sensitivity and specificity for detecting early epithelial cancer, compared to each modality applied independently. The ultimate goal of this line of research is to develop a novel optical imaging system and associated algorithms for noninvasive optical biopsy in oral cancer. Such a system may serve as a diagnostic and monitoring tool, a screening tool, a guide to standard biopsy and surgical intervention, and a tool for quantitative evaluation of therapeutic response. While this proposal is focused on the early detection of oral cancer we believe that the system and algorithms developed will also be generally applicable to many of the cancers of the epithelium. The following three specific aims will be addressed in this proposal: Aim 1: To develop a novel optical multimodality imaging system by combining OCT and FLIM technology for simultaneous micro-anatomical and biochemical imaging of tissue in vivo and ex vivo. Aim 2: To acquire a unique database of OCT/FLIM images and corresponding histopathology of the development of oral cancer in a hamster cheek pouch model. Aim 3: To develop new computational methods for OCT/FLIM image analysis and evaluate the sensitivity/specificity of the proposed multimodality imaging system for the early detection of oral neoplasia. The successful completion of this work will demonstrate the potential of combining OCT and FLIM as a means for non-invasive optical detection of oral pre-malignant and malignant lesions. PUBLIC HEALTH RELEVANCE: Early detection of epithelial oral cancer could significantly reduce cancer morbidity and mortality. Currently, the diagnosis of oral epithelial cancers is performed through direct or endoscopic visual inspection followed by biopsy. Since visual inspection is subjective and early pre-cancers are often not detectable under visual inspection, multiple biopsies have to be taken to increase the likelihood of finding a cancerous lesion. Therefore, techniques that can diagnose oral epithelial pre-cancer and cancer with higher accuracy than visual inspection alone are needed to guide tissue biopsy. Optical imaging is particularly well suited for imaging many of the epithelial tissues of the body including the oral cavity. Both biochemical and morphological changes accompany the transition from normal tissue to neoplastic tissue, yet the optical techniques heretofore investigated as potential screening and diagnostic tools for cancer have largely concentrated on one or the other. Here we propose to simultaneously collect both morphological and biochemical information by combining two well developed optical imaging techniques, optical coherence tomography (OCT) and fluorescence lifetime imaging (FLIM). The ultimate goal of this line of research is to develop a novel optical imaging system and associated algorithms which will enable noninvasive optical biopsy in oral cancer.

描述（由申请人提供）：口腔肿瘤变化的早期检测对提高生存率和生存者的生活质量具有很大的希望。光学成像的高空间分辨率可以使肿瘤病变的诊断成为可能，而肿瘤病变的大小仍然在亚毫米范围内，并且具有高度的可治疗性。生物化学和形态学变化都伴随着从正常组织到肿瘤组织的转变。然而，迄今为止作为癌症的潜在筛查和诊断工具而研究的光学技术主要集中在检测一种或另一种，主要是因为基本上缺乏容易提供两种类型信息的光学成像模式。在这里，我们建议同时收集形态和生化信息相结合的两个发达的光学成像技术，光学相干断层扫描（OCT）和荧光寿命成像（FLIM）。由于OCT和FLIM测量不同的组织特征（分别为结构和生物化学），因此我们假设与单独应用的每种模式相比，它们的协同作用将增加检测早期上皮癌的灵敏度和特异性。本研究的最终目标是开发一种新型的光学成像系统和相关算法，用于口腔癌的非侵入性光学活检。这样的系统可以用作诊断和监测工具、筛选工具、标准活检和手术干预的指南以及用于治疗反应的定量评估的工具。虽然该提议集中于口腔癌的早期检测，但我们相信所开发的系统和算法也将普遍适用于许多上皮癌。本提案将解决以下三个具体目标：目标1：通过结合OCT和FLIM技术开发一种新型光学多模态成像系统，用于体内和体外组织的同时显微解剖和生化成像。目标二：获取仓鼠颊囊模型中口腔癌发展的OCT/FLIM图像和相应组织病理学的独特数据库。目标三：开发OCT/FLIM图像分析的新计算方法，并评估拟议的多模态成像系统用于早期检测口腔肿瘤的灵敏度/特异性。这项工作的成功完成将证明结合OCT和FLIM作为口腔癌前病变和恶性病变的非侵入性光学检测手段的潜力。 公共卫生相关性：早期发现口腔上皮癌可以显著降低癌症发病率和死亡率。目前，口腔上皮癌的诊断是通过直接或内窥镜目视检查，然后进行活检。由于目视检查是主观的，并且在目视检查下通常无法检测到早期癌前病变，因此必须进行多次活检以增加发现癌性病变的可能性。因此，需要能够以比单独的视觉检查更高的准确性诊断口腔上皮癌前病变和癌症的技术来指导组织活检。光学成像特别适合于对包括口腔在内的身体的许多上皮组织进行成像。生物化学和形态学变化都伴随着从正常组织到肿瘤组织的转变，然而迄今为止作为癌症的潜在筛查和诊断工具而研究的光学技术主要集中在一个或另一个上。在这里，我们建议同时收集形态和生化信息相结合的两个发达的光学成像技术，光学相干断层扫描（OCT）和荧光寿命成像（FLIM）。这一系列研究的最终目标是开发一种新的光学成像系统和相关算法，这将使口腔癌的非侵入性光学活检。