Protein nucleation and crystallisation on novel 3-D templates

新型 3-D 模板上的蛋白质成核和结晶

• 批准号: BB/F004990/1
• 负责人: Daryl Williams
• 金额: $ 49.03万
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2008
• 资助国家: 英国
• 起止时间: 2008 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FF004990%2F1
• 关键词: Protein; nucleation; crystallisation; novel; templates

The crystallisation of biopharmaceuticals is poorly understood and is a rarely used commercial process for the primary separation and purification of proteins. This proposal describes a fundamental investigation into the nucleation and crystallisation of proteins via the development of novel solid templates with known surface chemistry and topography for their controlled heterogeneous nucleation and crystal growth. These templates will enhance the success rates for protein crystallisation/ nucleation, allowing an improved understanding of both processes as well as potentially forming the basis for the development of industrial bio-crystallisation processes. This proposal represents the first step towards a major new downstream opportunity for biopharmaceutical manufacturing, providing potentially major cost and productivity benefits in product separation and purification, as well as the major product stability and delivery advantages offered by crystalline products over traditional product/dosage forms. The ultimate success of this strategy would give the UK industry an international competitive advantage in biopharmaceutical processing.

生物药物的结晶是知之甚少，是一个很少使用的初步分离和纯化蛋白质的商业过程。该提案描述了一个基本的调查成核和结晶的蛋白质通过开发新的固体模板与已知的表面化学和地形为他们控制的异质成核和晶体生长。这些模板将提高蛋白质结晶/成核的成功率，从而提高对这两个过程的理解，并可能为工业生物结晶过程的发展奠定基础。该提案代表了生物制药制造的主要新下游机会的第一步，在产品分离和纯化方面提供了潜在的主要成本和生产率优势，以及结晶产品相对于传统产品/剂型提供的主要产品稳定性和递送优势。这一战略的最终成功将使英国工业在生物制药加工方面具有国际竞争优势。

项目成果

Effects of Oscillatory Flow on the Nucleation and Crystallization of Insulin

振荡流对胰岛素成核和结晶的影响

• DOI: 10.1021/cg200158z
• 发表时间: 2011
• 期刊: Crystal Growth & Design
• 影响因子: 3.8
• 作者: Parambil J

Selective Crystallization of Proteins Using Engineered Nanonucleants

• DOI: 10.1021/cg201443s
• 发表时间: 2012-03-01
• 期刊: CRYSTAL GROWTH & DESIGN
• 影响因子: 3.8
• 作者: Shah, Umang V.;Williams, Daryl R.;Heng, Jerry Y. Y.
• 通讯作者: Heng, Jerry Y. Y.

Protein Crystallization by Forced Flow through Glass Capillaries: Enhanced Lysozyme Crystal Growth

通过玻璃毛细管强制流动进行蛋白质结晶：增强溶菌酶晶体生长

• DOI: 10.1021/cg900492j
• 发表时间: 2010
• 期刊: Crystal Growth & Design
• 影响因子: 3.8
• 作者: Roberts M

Effect of surface chemistry of novel templates on crystallization of proteins

• DOI: 10.1016/j.ces.2012.01.049
• 发表时间: 2012-07-30
• 期刊: CHEMICAL ENGINEERING SCIENCE
• 影响因子: 4.7
• 作者: Tsekova, Daniela S.;Williams, Daryl R.;Heng, Jerry Y. Y.
• 通讯作者: Heng, Jerry Y. Y.

Crystallization of Proteins at Ultralow Supersaturations Using Novel Three-Dimensional Nanotemplates

• DOI: 10.1021/cg201190c
• 发表时间: 2012-04-01
• 期刊: CRYSTAL GROWTH & DESIGN
• 影响因子: 3.8
• 作者: Shah, Umang V.;Allenby, Mark C.;Heng, Jerry Y. Y.
• 通讯作者: Heng, Jerry Y. Y.