Plasmonic Detection of Free-Bilirubin in Neonates
新生儿游离胆红素的等离子体检测
基本信息
- 批准号:7417780
- 负责人:
- 金额:$ 13.27万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2007
- 资助国家:美国
- 起止时间:2007-05-01 至 2010-04-30
- 项目状态:已结题
- 来源:
- 关键词:AdultApplications GrantsAreaAwardBackBilirubinBindingBiologicalBiological AssayBiomedical EngineeringBiotechnologyBloodBlood - brain barrier anatomyCalibrationChemicalsClinicalClinical ResearchClinical TrialsColloidsDependenceDetectionDiffuseDisciplineEnzyme-Linked Immunosorbent AssayFaceFilmFluorescenceGestational AgeGlassGoldHealthHealth SciencesHousingHumanIcterusIslandJournalsLabelLaboratoriesLinkLocalizedMeasurementMeasuresMetalsMethodsMolecular WeightNanostructuresNeonatalNeurologicOperative Surgical ProceduresPeer ReviewPhotonsPlasticsPolymerase Chain ReactionPolymersPremature InfantProcessPublicationsPurposeRNAResearchResearch Project GrantsRiskSamplingScreening procedureSerumSerum AlbuminSilicon DioxideSilverSlideStagingStandards of Weights and MeasuresSurfaceSystemTechnologyTestingThickThinkingTimeTodayToxic effectTranslatingUnited States Food and Drug AdministrationUnited States National Institutes of HealthUniversitiesWeekWhole Bloodbasecopolymerdesigndetectorfluorophorehydrophilicityimprovedinstrumentmultidisciplinarynanoparticleneonatenervous system disordernew technologynovelpostnatalquantum
项目摘要
DESCRIPTION (provided by applicant): Over the last couple of years our laboratories have developed a new fluorescence based phenomenon, which is now having profound implications in clinical biotechnology. This new technology has led to the recent publication of over 50 peer reviewed articles, one edited hard bound volume and the launch of a new peer reviewed Springer Journal, entitled Plasmonics. This new phenomenon relies on the interactions of fluorophores with metallic nanoparticles and can increase the quantum yield of fluorescing species, provide spatially localized excitation and can even substantially improve fluorophore photostability. Our new technology clearly demonstrates that these novel effects can result in up to a million-fold more photons per fluorophore, which when applied to assay sensing platforms, provides for quick and simple high sensitivity assays. In this regard, under a recently completed NIH award to the PI, we were able to develop an enhanced assay platform for RNA target detection which has the sensitivity of PCR and ELISA, but most profoundly, without any amplification steps. In this R21 proposal we have assembled a multidisciplinary team of Spectroscopists, Biostatisticians and Clinicians alike, to develop a high sensitivity assay for the detection of free unbound Bilirubin in neonatal serum, an area of intense research and high clinical importance due to its ability to cross the blood brain barrier and participate in various neurological disorders. At present, tests for free unbound bilirubin are arduous, time consuming, and no approach has to date been standardized. The Metal-Enhanced Fluorescence (MEF) free bilirubin assay described here, offers high sensitivity detection, simple operation, can be made disposable and has the potential to even be standardized in laboratories around the world. The MEF assay functions as follows: Human Serum Albumin (HSA), which binds and transports serum free and bound bilirubin respectively, is immobilized in a hydrophilic copolymer, itself immobilized onto a glass slide. When serum, containing serum albumin predominantly bound bilirubin (> 99 %) is washed across the assay surface, free bilirubin diffuses into the polymer, binds to the embedded HSA and becomes intrinsically highly fluorescent due to its locality to surface silver nanostructures. We intend to optimize and determine the sensitivity of the assay using both laboratory and clinical samples and most importantly, statistically compare our results with the "UB analyzer", one of only 3 instruments in the USA designed to measure free bilirubin. This proposed research aims to develop a highly novel sensing platform for whole blood free Bilirubin detection in neonates. No commercial instrument is available in the US today. Given that free bilirubin has been linked to later stage neurological disorders, then the technology underpinned by this proposal goes some significant ways to safeguard both neonatal and in-turn adult health.
描述(由申请人提供):在过去的几年中,我们的实验室已经开发出一种新的基于荧光的现象,现在在临床生物技术中具有深远的意义。这项新技术导致最近出版了50多篇同行评议的文章,一本经过编辑的精装本,并推出了一本新的同行评议的施普林格杂志,题为等离子体学。这种新现象依赖于荧光团与金属纳米粒子的相互作用,可以增加荧光物质的量子产率,提供空间局部激发,甚至可以大大提高荧光团的光稳定性。我们的新技术清楚地表明,这些新效应可以导致每个荧光团的光子增加多达一百万倍,当应用于分析传感平台时,提供快速简单的高灵敏度分析。在这方面,根据最近完成的NIH对PI的奖励,我们能够开发出一种增强的RNA靶检测分析平台,该平台具有PCR和ELISA的敏感性,但最重要的是,无需任何扩增步骤。在这项R21提案中,我们组建了一个由光谱学家、生物统计学家和临床医生组成的多学科团队,开发一种用于检测新生儿血清中游离未结合胆红素的高灵敏度检测方法。由于胆红素能够穿过血脑屏障并参与各种神经系统疾病,这是一个研究领域,具有很高的临床重要性。目前,游离未结合胆红素的检测是艰巨的,耗时的,迄今为止还没有标准化的方法。本文描述的金属增强荧光(MEF)游离胆红素测定具有高灵敏度检测,操作简单,可以一次性使用,甚至有可能在世界各地的实验室中标准化。MEF检测的功能如下:人血清白蛋白(HSA)分别结合和运输游离血清胆红素和结合血清胆红素,固定在亲水性共聚物中,自身固定在玻璃载玻片上。当含有血清白蛋白为主结合的胆红素(bbb99%)的血清在实验表面洗涤时,游离胆红素扩散到聚合物中,与嵌入的HSA结合,并由于其在表面银纳米结构上的位置而具有本质上的高荧光。我们打算利用实验室和临床样品来优化和确定检测的灵敏度,最重要的是,将我们的结果与“UB分析仪”进行统计比较,UB分析仪是美国仅有的3种用于测量游离胆红素的仪器之一。本研究旨在开发一种全新的检测新生儿全血游离胆红素的传感平台。今天在美国没有商业工具可用。鉴于游离胆红素与晚期神经系统疾病有关,那么这项提议所支持的技术在保护新生儿和成人健康方面具有重要意义。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
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Christopher Darren Geddes其他文献
Christopher Darren Geddes的其他文献
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{{ truncateString('Christopher Darren Geddes', 18)}}的其他基金
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- 批准号:
8466281 - 财政年份:2012
- 资助金额:
$ 13.27万 - 项目类别:
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- 资助金额:
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- 资助金额:
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7670042 - 财政年份:2009
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- 批准号:
7678811 - 财政年份:2008
- 资助金额:
$ 13.27万 - 项目类别:
Plasmonic Detection of Free-Bilirubin in Neonates
新生儿游离胆红素的等离子体检测
- 批准号:
7313027 - 财政年份:2007
- 资助金额:
$ 13.27万 - 项目类别:
Plasmonic Detection of Free-Bilirubin in Neonates
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7555459 - 财政年份:2007
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7181997 - 财政年份:2005
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NOBLE-METAL NANOSTRUCTURES FOR METAL-ENHANCED FLUORESCENCE
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