The Ciona savignyi Genetic Map

此拨款是针对基因和基因组计划公告工具而提交的 新兴模式生物研究。我建议在海鞘中构建一个300标记的连锁图谱， Ciona savignyi，它与将成为注释的高质量基因组参考完全集成 序列。这一图谱不仅有助于突变的克隆，而且还有助于实现远距离的 有效执行全基因组所需的萨维尼弧菌参考序列中的邻接性 扫描诱导或自然发生的突变。除了全基因组图谱，高分辨率的精细- 将进行不确定序列组装区域的比例映射，以解决C中的歧义。 萨维尼人基因组参考序列。这张地图，以及一个包含数千个潜在 分布在整个基因组中的多态标记将被提供给社区。 海鞘是最简单的脊索动物之一，但它们的基本发育机制是 在结构和分子上与脊椎动物的保守。尽管保护水平很高， 海鞘动物的基因组在大小上与果蝇的相当(-180Mb)，因此在实验上表现出 有利的基因组简单性。此外，海鞘有许多诱人的胚胎学和实验 有助于研究发育过程中的基因调控的特征。在过去的几年里，C.savignyi 已经发展成为一种遗传模式生物，突变株很容易从野生种群或 通过诱变。遗传图谱、标记和基因组组装的远程邻接性是 缺少的、极其重要的资源，以建立一个完整的遗传和基因组萨维尼 模型生物。 萨维尼乔纳和它的姊妹种，肠乔纳，是独一无二的信息，关于 脊索胚胎发育的机制。胚胎发育的基本特征和具体特征 人类和这些脊索动物之间的发育和基因调控是保守的。洞察世界 以Ciona为模型的调控和发育的分子机制是高效的和 与人类的同源过程有关。

This grant is submitted in response to the Program Announcement Tools for Genetic and Genomic Studies in Emerging Model Organisms. I propose to construct a 300-marker linkage map in the ascidian, Ciona savignyi, that is fully integrated with what will become the annotated high quality genome reference sequence. This map will not only aid in the cloning of mutations, but also in achieving the long range contiguity in the C. savignyi reference sequence that will be needed to efficiently perform whole genome scans for induced or naturally occurring mutations. In addition to the genome-wide map, high-resolution fine- scale mapping in regions of uncertain sequence assembly will be performed to resolve ambiguities in the C. savignyi genome reference sequence. The map, along with a database of thousands of potentially polymorphic markers distributed across the genome, will be made available to the community. Ascidians are among the simplest chordates, yet their fundamental developmental mechanisms are structurally and molecularly conserved with those of vertebrates. Despite the high level of conservation, ascidian genomes are comparable in size to Drosophila's (-180 Mb), thus exhibiting experimentally advantageous genomic simplicity. Further, ascidians have many attractive embryological and experimental features that facilitate studies of gene regulation during development. Over the past few years, C. savignyi has been developed as a genetic model organism, with mutants readily obtainable from wild populations or by mutagenesis. The genetic map,the markers, and the long-range contiguity of the genome assembly are the missing, critically important, resources to establish C. savignyi as a full-fledged genetic and genomic model organism. Ciona savignyi and its sister species, Ciona intestinalis, are uniquely informative with regards to the mechanisms of chordate embryonic development. Fundamental as well as specific features of embryonic development and gene regulation are conserved between humans and these chordates. Insights into the molecular mechanisms of regulation and development using Ciona as a model are highly effective and relevant to the homologous processes in human.