Multicellular recording system to investigate central nervous system dynamics

研究中枢神经系统动力学的多细胞记录系统

• 批准号: BB/F011415/1
• 负责人: Evelyne Sernagor
• 金额: $ 10.9万
• 依托单位: Newcastle University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2008
• 资助国家: 英国
• 起止时间: 2008 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FF011415%2F1
• 关键词: Multicellular; recording; system; investigate; central

In the brain, specific neural activity patterns arise from the simultaneous activation of thousands or millions of neurones interconnected in a very specific way, conveying unique properties to the entire network. Therefore, if we want to investigate how neurones communicate with each other, we need to record from many cells simultaneously. Thanks to new technologies, this is now possible. Impulses generated by neurones can be recorded with electrodes that detect electrical activity. Large arrays of many of these electrodes are now available, enabling us to record simultaneously from dozens of sites. Optical recordings, on the other hand, allow us to visualise changes in the concentration of various ions that are involved in the control of neural activity, while they are flowing across the membrane of the active neurones. These ionic fluxes can be linked either to neural excitation or inhibition and are therefore not necessarily reflecting impulse generation. Taken together, the combination of electrical and optical recordings over large-scale neuronal networks provides a powerful tool to help us understand the mechanisms of neuronal communication in much greater detail. In this proposal, we intend to establish a state-of-the-art system that will allow us to perform electrical recordings from a 60-electrode array at the same time as high resolution imaging of neuronal activity. The system will be used for several projects, ranging from investigating the behaviour of embryonic retinal networks to the generation and control of cortical and hippocampal oscillations associated with various cognitive states. Moreover, it will also represent an invaluable experimental tool to help in the design of a device that has the huge potential to become the platform for the development of a retinal implant that could help restore impaired visual perception following retinal dystrophic diseases.

在大脑中，特定的神经活动模式来自以非常特定的方式相互连接的数千个或数百万个神经元的同时激活，向整个网络传达独特的属性。因此，如果我们想要研究神经元是如何相互通信的，我们需要同时从多个细胞进行记录。多亏了新技术，这现在成为可能。神经元产生的脉冲可以用检测电活动的电极记录下来。其中许多电极的大阵列现已可用，使我们能够同时从数十个地点进行记录。另一方面，光学记录使我们能够可视化涉及神经活动控制的各种离子浓度的变化，当它们流经活跃神经元的膜时。这些离子通量可以与神经兴奋或抑制联系在一起，因此不一定反映脉冲的产生。综上所述，大规模神经元网络上的电和光记录的结合为我们提供了一个强大的工具来帮助我们更详细地了解神经元通信的机制。在这项提案中，我们打算建立一个最先进的系统，使我们能够在对神经元活动进行高分辨率成像的同时，从60个电极阵列进行电记录。该系统将用于几个项目，从研究胚胎视网膜网络的行为到产生和控制与各种认知状态相关的皮质和海马体振荡。此外，它还将是一个宝贵的实验工具，有助于设计一种设备，这种设备具有巨大的潜力，可以成为开发视网膜植入物的平台，这种植入物可以帮助恢复视网膜营养不良疾病后受损的视觉感知。

项目成果

A data repository and analysis framework for spontaneous neural activity recordings in developing retina.

• DOI: 10.1186/2047-217x-3-3
• 发表时间: 2014-03-26
• 期刊: GigaScience
• 影响因子: 9.2
• 作者: Eglen SJ;Weeks M;Jessop M;Simonotto J;Jackson T;Sernagor E
• 通讯作者: Sernagor E

Blockade of pathological retinal ganglion cell hyperactivity improves optogenetically evoked light responses in rd1 mice.

• DOI: 10.3389/fncel.2015.00330
• 发表时间: 2015
• 期刊: Frontiers in cellular neuroscience
• 影响因子: 5.3
• 作者: Barrett JM;Degenaar P;Sernagor E
• 通讯作者: Sernagor E

Carbon nanotube electrodes for retinal implants: A study of structural and functional integration over time.

• DOI: 10.1016/j.biomaterials.2016.10.018
• 发表时间: 2017-01
• 期刊: Biomaterials
• 影响因子: 14
• 作者: Eleftheriou CG;Zimmermann JB;Kjeldsen HD;David-Pur M;Hanein Y;Sernagor E
• 通讯作者: Sernagor E

Age-dependent homeostatic plasticity of GABAergic signaling in developing retinal networks.

• DOI: 10.1523/jneurosci.3112-11.2011
• 发表时间: 2011-08-24
• 期刊: The Journal of neuroscience : the official journal of the Society for Neuroscience
• 作者: Hennig MH;Grady J;van Coppenhagen J;Sernagor E
• 通讯作者: Sernagor E