Release of Apoptogenic Proteins from Brain Mitochondria

脑线粒体释放凋亡蛋白

• 批准号: 7340745
• 负责人: Nickolay Brustovetsky
• 金额: $ 25.94万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-12-01 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7340745
• 关键词: ATP sensitive potassium channel complex; Adenine Nucleotide Translocase; Antioxidants; Apoptosis; Apoptotic; BAX gene; Bax protein; Brain; Brain Edema; Caspase; Cell Death; Cells; Cessation of life; DNA; Diagnostic; Foundations; Generations; Goals; Inner mitochondrial membrane; Knowledge; Laboratories; Lead; Lipid Peroxidation; Liposomes; Membrane; Mitochondria; Mitochondrial Proteins; Molecular; Nerve Degeneration; Neurons; Organelles; Outer Mitochondrial Membrane; Permeability; Phospholipase A2; Phospholipases A; Phospholipids; Positioning Attribute; Potassium Channel; Process; Protein Family; Proteins; Reactive Oxygen Species; Recombinants; Research; Research Personnel; Role; Rupture; Stroke; Swelling; Testing; Therapeutic; Work; apoptosis inducing factor; cytochrome c; design; endonuclease G; experience; inhibitor/antagonist; member; mitochondrial K(ATP) channel; nervous system disorder; neuron apoptosis; neuropathology; novel; phospholipase A2 inhibitor; pro-apoptotic protein; programs; tool

DESCRIPTION (provided by applicant): There is a fundamental gap in our understanding of the mechanisms of the release of mitochondrial apoptogenic factors induced by elevated Ca2+ and by pro-apoptotic proteins. Our long-term goal is to establish the role of mitochondria in neuronal apoptosis. The objective of this study is to delineate the mechanisms of release of apoptogenic proteins from brain mitochondria initiated by elevated Ca2+ or by pro-apoptotic proteins. The central hypothesis of the proposed research is that an increased generation of reactive oxygen species, augmentation of lipid peroxidation, activation of phospholipase A2, and K+ influx in brain mitochondria are the major processes leading to the release of apoptogenic proteins induced by elevated Ca2+ or pro-apoptotic proteins tBID and BAX. In Specific Aim 1 we will establish K+-dependent mechanisms of the Ca2+-induced swelling of brain mitochondria and release of apoptogenic proteins. Inhibitors of mitochondrial K+ channels and the adenine nucleotide translocase will be applied to isolated brain mitochondria or to cultured neurons to establish their role in the Ca2+-induced swelling, and release of apoptogenic proteins. In Specific Aim 2 we will determine the extent to which an activation of mitochondrial K+ channels and the permeability transition contributes to the release of apoptogenic factors induced by pro-apoptotic proteins tBID and BAX. Inhibitors of the permeability transition and blockers of K+ channels will be used to identify their role in the release of the apoptogenic proteins. In Specific Aim 3 we will establish the role of reactive oxygen species, lipid peroxidation and phospholipase A2 in the release of apoptogenic proteins induced by tBID and BAX. Various antioxidants and inhibitors of phospholipase A2 will be used to inhibit the release of apoptogenic proteins. In Specific Aim 4 we will determine the role of caspases in the release of apoptogenic proteins from brain mitochondria exposed to tBID and BAX. Isolated brain mitochondria exposed to tBID and BAX and treated with recombinant caspases will be used to test this hypothesis. The proposed research lays the foundation for a better understanding of the molecular mechanisms of the permeabilization of the outer mitochondrial membrane induced by elevated Ca 2+ or pro-apoptotic proteins tBID and BAX and contributes to filling in a gap in our knowledge of these phenomena.

描述（由申请人提供）：我们对高 Ca2+ 和促凋亡蛋白诱导的线粒体凋亡因子释放机制的理解存在根本性差距。我们的长期目标是确定线粒体在神经细胞凋亡中的作用。本研究的目的是阐明由 Ca2+ 升高或促凋亡蛋白引发的脑线粒体释放凋亡蛋白的机制。该研究的中心假设是，活性氧生成的增加、脂质过氧化的增强、磷脂酶 A2 的激活以及脑线粒体中 K+ 的流入是导致 Ca2+ 或促凋亡蛋白 tBID 和 BAX 升高诱导的凋亡蛋白释放的主要过程。在具体目标 1 中，我们将建立 Ca2+ 诱导脑线粒体肿胀和凋亡蛋白释放的 K+ 依赖性机制。线粒体 K+ 通道和腺嘌呤核苷酸转位酶抑制剂将应用于分离的脑线粒体或培养的神经元，以确定它们在 Ca2+ 诱导的肿胀和凋亡蛋白释放中的作用。在具体目标 2 中，我们将确定线粒体 K+ 通道的激活和通透性转变对促凋亡蛋白 tBID 和 BAX 诱导的凋亡因子释放的贡献程度。通透性转变抑制剂和 K+ 通道阻断剂将用于确定它们在凋亡蛋白释放中的作用。在具体目标 3 中，我们将确定活性氧、脂质过氧化和磷脂酶 A2 在 tBID 和 BAX 诱导的凋亡蛋白释放中的作用。各种抗氧化剂和磷脂酶A2抑制剂将用于抑制凋亡蛋白的释放。在特定目标 4 中，我们将确定半胱天冬酶在暴露于 tBID 和 BAX 的脑线粒体释放凋亡蛋白中的作用。 暴露于 tBID 和 BAX 并用重组半胱天冬酶处理的分离脑线粒体将用于检验这一假设。该研究为更好地理解 Ca 2+ 或促凋亡蛋白 tBID 和 BAX 升高诱导的线粒体外膜透化的分子机制奠定了基础，并有助于填补我们对这些现象的认识空白。