IDENTIFICATION OF PUTATIVE PROTEIN CANDIDATES IN THE EXPORT MECHANISM OF ANNEXI

附件出口机制中推定候选蛋白质的鉴定

• 批准号: 7355101
• 负责人: KATHERINE AMBERSON HAJJAR
• 金额: $ 0.25万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7355101
• 关键词: IDENTIFICATION; PUTATIVE; PROTEIN; CANDIDATES; EXPORT

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Annexin 2 is a profibrinolytic co-receptor for plasminogen and tissue plasminogen activator that stimulates activation of the major fibrinolysin, plasmin, at cell surfaces. In human subjects, over-expression of annexin 2 in acute promyelocytic leukemia leads to a bleeding diathesis reflective of excessive cell surface annexin 2-dependent generation of plasmin. In addition, mice completely deficient in annexin 2 display fibrin accumulation within blood vessels and impaired clearance of injury-induced thrombi. We have previously shown that endothelial cell annexin 2, a protein that lacks a typical signal peptide, translocates from the cytoplasm to the extracytoplasmic plasma membrane in response to brief temperature stress both in vitro and in vivo in the absence of cell death or cell lysis. This regulated response is independent of new protein or mRNA synthesis and does not require the classical endoplasmic reticulum¿Golgi pathway. Translocation of annexin 2 induced by temperature stress is dependent on both expression of protein p11 (S100A10) and tyrosine phosphorylation of annexin 2 because the release of annexin 2 is completely eliminated on depletion of p11, inactivation of tyrosine kinase, or mutation of tyrosine 23. Translocation of annexin 2 to the cell surface dramatically increases tissue plasminogen activator-dependent plasminogen activation potential and may represent a novel stress-induced protein secretion pathway. We have identified by immunoaffinity assays and mass spectrometry at least four proteins that may be associated with the translocation mechanism of annexin 2 to the cell surface. These putative protein candidates are currently being investigated.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。膜联蛋白 2 是纤溶酶原和组织纤溶酶原激活剂的纤溶原共受体，可刺激细胞表面主要纤溶酶（纤溶酶）的激活。在人类受试者中，急性早幼粒细胞白血病中膜联蛋白 2 的过度表达会导致出血素质，这反映了细胞表面膜联蛋白 2 依赖性纤溶酶的过度生成。此外，完全缺乏膜联蛋白2的小鼠表现出血管内的纤维蛋白积聚以及损伤引起的血栓的清除受损。我们之前已经证明，在没有细胞死亡或细胞裂解的情况下，内皮细胞膜联蛋白 2（一种缺乏典型信号肽的蛋白质）在体外和体内响应短暂的温度应激，从细胞质易位到胞质外质膜。这种调节反应独立于新蛋白质或 mRNA 合成，并且不需要经典的内质网-高尔基体途径。温度应激诱导的膜联蛋白 2 易位取决于蛋白 p11 (S100A10) 的表达和膜联蛋白 2 的酪氨酸磷酸化，因为膜联蛋白 2 的释放在 p11 耗尽、酪氨酸激酶失活或酪氨酸 23 突变时完全消除。膜联蛋白 2 易位到细胞表面会显着增加组织纤溶酶原激活剂依赖性纤溶酶原 激活潜力，可能代表一种新的应激诱导的蛋白质分泌途径。我们通过免疫亲和测定和质谱鉴定了至少四种可能与膜联蛋白 2 向细胞表面易位机制相关的蛋白质。目前正在研究这些假定的候选蛋白质。