BACTERIAL STUDIES

细菌研究

• 批准号: 7355285
• 负责人: DAVID L POPHAM
• 金额: $ 0.09万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-02-01 至 2007-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7355285
• 关键词: BACTERIAL; STUDIES

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Bacillus subtilis Projects Polymerization of the bacterial peptidoglycan cell wall has proven to be one of the best targets for antibiotics because it is essential for the cells, is highly conserved across essentially all bacterial genera, and takes place outside of the cell, where the antibiotics have easy access. A greater understanding of the details of peptidoglycan polymerization will contribute to the development of new antibiotics. With funding from a grant from the NIH, we study many aspects of peptidoglycan synthesis in B. subtilis. These include: 1) The roles played by the 16 different penicillin-binding proteins (PBPs) in putting together vegetative cell walls and spore cell walls of the proper shape, size and structure; 2) The protein-protein interactions made by the major PBPs; 3) Regulation of the timing of spore peptidoglycan synthesis; and 4) The relationship of spore peptidoglycan structure and spore resistance properties. Bacillus anthracis Projects The spores that cause Anthrax can lie in a dormant, extremely stable state for many years. In addition, these spores are highly resistant to most treatments commonly used for disinfection. Once the spores enter a host body, they must germinate and return to a growing state in order to cause disease. This germination also renders the spores sensitive to disinfection agents. With funding from a grant from the NIH, we are determining the enzymatic activities required for degradation of the B. anthracis spore peptidoglycan wall, an essential step in spore germination. With an understanding of the proteins involved in germination and the mechanism that triggers this process, we may be able to design ways to either prevent or stimulate germination. This can lead to better methods of infection control and cleanup of contaminated sites.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。细菌肽聚糖细胞壁的聚合已被证明是抗生素的最佳靶点之一，因为它对细胞至关重要，在几乎所有细菌属中高度保守，并且发生在细胞外，抗生素很容易进入。更深入地了解肽聚糖聚合的细节将有助于开发新的抗生素。在美国国立卫生研究院的资助下，我们研究了B中肽聚糖合成的许多方面。枯草杆菌。其中包括：1）16种不同的青霉素结合蛋白（PBP）在组装营养细胞壁和孢子细胞壁中的作用; 2）主要PBP的蛋白质-蛋白质相互作用; 3）孢子肽聚糖合成时间的调节; 4）孢子肽聚糖结构与孢子抗性的关系。 炭疽芽孢杆菌项目引起炭疽的孢子可以处于休眠状态，非常稳定的状态多年。此外，这些孢子对通常用于消毒的大多数处理具有高度抗性。一旦孢子进入宿主体内，它们必须发芽并恢复生长状态以引起疾病。这种萌发也使孢子对消毒剂敏感。在美国国立卫生研究院的资助下，我们正在确定降解B所需的酶活性。炭疽菌孢子的肽聚糖壁，孢子萌发的一个重要步骤。了解了与发芽有关的蛋白质和触发这一过程的机制，我们也许能够设计出防止或刺激发芽的方法。这可以导致更好的感染控制和污染场地清理方法。