SPORE PEPTIDOGLYCAN SYNTHESIS IN BACILLUS SUBTILIS

枯草芽孢杆菌孢子肽聚糖的合成

• 批准号: 6345243
• 负责人: DAVID L POPHAM
• 金额: $ 0.93万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6345243
• 关键词: biological products; biomedical resource; immunology; infection; inflammation; reproductive system; spectrometry; structural biology; technology /technique; vaccines; virus

The incidence of gonorrhea has decreased during the last decade. In stark contrast to this downward trend, the proportion of strains showing drug resistance has increased. Penicillin resistant strains were first reported in 1976 and, since that time, drug resistant Neisseria gonorrhoeae isolates from various parts of the world have been described. Although some quinolones and extended-spectrum cephalosporins are currently proving adequate for a treatment regimen, species vulnerability will likely diminish and antimicrobial resistance is a matter of time. Physiological adaptability and evasion of immune host response contribute to microbial persistence and many of these strategies can be manifested through and by the components of the outer surface Hpooligosaccharide (LOS). Foremost, and probably related, are the demonstrable antigenic variations and oligosaccharide (OS) heterogeneity. Transparent to a significant part of these differences is the mAb 2C7, which recognizes an OS epitope that is widely expressed in vivo. A better understanding of the structural features of reactivity for 2C7 may provide an improved basis for gonococcal vaccine development. In this investigation, using several known andone unknown LOS, we are carrying out their structural characterization using simple chemical manipulations and the mass spectrometry techniques of electrospray ionization, collision-induced decomposition tandem mass spectrometry.

淋病的发病率在过去十年中有所下降。 与这种下降趋势形成鲜明对比的是， 表明抗药性增加了 青霉素耐药菌株 1976年首次报道，从那时起， 来自世界各地的淋病奈瑟菌分离株具有 被描述。 虽然一些喹诺酮类药物和超广谱抗生素 头孢菌素目前被证明对于治疗方案是足够的， 物种的脆弱性可能会减少， 抵抗只是时间问题。 生理适应性和 逃避宿主免疫应答有助于微生物的持久性 其中许多策略可以通过 外表面的Hpooligosaccharide（LOS）。 首先， 并且可能相关的是可证明的抗原变异， 寡糖（OS）异质性。 对重要部分透明 这些差异之一是mAb 2C7，其识别OS表位 在体内广泛表达。 更好地理解 2C7的反应性的结构特征可以提供改进的 淋球菌疫苗研制的基础。 在这次调查中， 使用几个已知的和一个未知的LOS，我们正在执行他们的 使用简单的化学操作和 电喷雾电离的质谱技术， 碰撞诱导分解串联质谱法。