INTERACTIONS OF APE1 TO C-JUN OR E3330

APE1 与 C-JUN 或 E3330 的相互作用

• 批准号: 7355301
• 负责人: Millie M Georgiadis
• 金额: $ 0.05万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-02-01 至 2007-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7355301
• 关键词: INTERACTIONS; APE1; JUN; E3330

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Ape1 (Ape(delta)40) is a protein that participates in redox regulation of transcription factor function. The redox function of Ape1 involves reduction of oxidized cysteine residues within the DNA binding domains of several transcription factors, including c-Jun. E3330 is a small molecule containing a quinone. It binds directly to Ape1 and inhibits the redox function of Ape1. E3330 blocks the ability of Ape1 to reduce c-Jun. We wish to use PLIMSTEX (Protein-Ligand Interaction by MS, Titration and H/D exchange) to study the interactions of Ape1 to c-Jun or E3330. The method would enable us to determine the binding affinity constants between Ape1 and c-Jun or E3330 and perhaps to map the site of interaction of E3330 and Ape1.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。Ape 1（Ape（delta）40）是一种参与转录因子功能的氧化还原调节的蛋白质。Ape 1的氧化还原功能涉及几种转录因子（包括c-Jun）的DNA结合结构域内的氧化半胱氨酸残基的还原。E3330是含有醌的小分子。它直接与Ape 1结合并抑制Ape 1的氧化还原功能。E3330阻断Ape 1还原c-Jun的能力。我们希望使用PLIMSTEX（Protein-Ligand Interaction by MS，Titration and H/D exchange）来研究Ape 1与c-Jun或E3330的相互作用。该方法将使我们能够确定Ape 1和c-Jun或E3330之间的结合亲和力常数，并可能映射E3330和Ape 1相互作用的位点。