INTERACTIONS OF APE1 TO C-JUN OR E3330

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。APE1(APE(Delta)40)是一种参与氧化还原调节转录因子功能的蛋白质。APE1的氧化还原功能包括还原几种转录因子DNA结合域中的氧化半胱氨酸残基，包括c-jun。E3330是一种含有苯二酚的小分子。它直接与APE1结合，抑制APE1的氧化还原功能。E3330阻断APE1降低c-jun的能力。我们希望用PLIMSTEX(蛋白质-配体相互作用的MS，滴定和H/D交换)来研究APE1与c-jun或E3330的相互作用。该方法将使我们能够确定APE1与c-jun或E3330之间的结合亲和力常数，并可能定位E3330与APE1的相互作用部位。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Ape1 (Ape(delta)40) is a protein that participates in redox regulation of transcription factor function. The redox function of Ape1 involves reduction of oxidized cysteine residues within the DNA binding domains of several transcription factors, including c-Jun. E3330 is a small molecule containing a quinone. It binds directly to Ape1 and inhibits the redox function of Ape1. E3330 blocks the ability of Ape1 to reduce c-Jun. We wish to use PLIMSTEX (Protein-Ligand Interaction by MS, Titration and H/D exchange) to study the interactions of Ape1 to c-Jun or E3330. The method would enable us to determine the binding affinity constants between Ape1 and c-Jun or E3330 and perhaps to map the site of interaction of E3330 and Ape1.