RADIOLOGICAL RESEARCH ACCELERATOR FACILITY (RARAF)

放射学研究加速器设施 (RARAF)

• 批准号: 7118253
• 负责人: DAVID JONATHAN BRENNER
• 金额: $ 88.42万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-30 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7118253
• 关键词: bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical automation; biomedical equipment; biomedical equipment purchase; biomedical resource; electromagnetic radiation; particle accelerators; radiation detector; radiography; single cell analysis; technology /technique development

DESCRIPTION (provided by applicant): The technology development component focuses on four areas which our users have identified as ripe for enhancement: improved accuracy, enhanced imaging capabilities, extension of the charged-particle microbeam to X rays; and development of a simple stand-alone microbeam that can be used without physics support and can be potentially exported to other labs. A highlight regarding improved accuracy and reliability is that we have just received a $2M NIH high-end equipment grant (plus institutional support) for a new particle accelerator, central to the microbeam facility, which will be installed under the auspices of this P-41 grant. Highlights regarding enhanced imaging and sensor technology will be an on-line custom multi-photon imaging system to allow time-lapse fluorescent imaging of early (scale of seconds to minutes) radiation events in individual live cells, a new imaging system based on quantitative phase microscopy to image live cells and cell nuclei without use of potentially cytotoxic stains, and a system for single-cell microsensors to monitor the flux of molecules such as oxygen and NO, in microbeam-irradiated individual cells. In collaboration with a variety of investigators from outside and within Columbia University, 20 collaborative and service-based research projects are described, which both drive and utilize the new resources. All are logical outgrowths of current federally funded research In addition to the ongoing training of undergraduates, graduate students, and post docs, training will be enhanced through formal programs for high school students, high school teachers, and undergraduates. In addition to dissemination of material through talks, our website, and peer-reviewed publications, we will run a 2nd International Workshop "Imaging and Probing Individual Cells: Application to Signaling, Structure and Function", as well as the biannual "International Workshop on Microbeam Probes of Cellular Radiation Response".

描述（由申请人提供）：技术开发组件侧重于我们用户确定为增强功能的四个领域：提高准确性，增强的成像功能，将带电的粒子Microbeam扩展到X Rays；以及可以在没有物理支持的情况下使用的简单独立微膜的开发，并可能导出到其他实验室。 关于提高准确性和可靠性的亮点是，我们刚刚为新的粒子加速器获得了200万美元的NIH高端设备拨款（加机构支持），这是Microbeam设施的中心，该设施将在此P-41赠款的主持下安装。 Highlights regarding enhanced imaging and sensor technology will be an on-line custom multi-photon imaging system to allow time-lapse fluorescent imaging of early (scale of seconds to minutes) radiation events in individual live cells, a new imaging system based on quantitative phase microscopy to image live cells and cell nuclei without use of potentially cytotoxic stains, and a system for single-cell microsensors to monitor the flux of molecules such as oxygen and不，在微氨基辐照的单个细胞中。 与来自哥伦比亚大学外部和外部的各种调查人员合作，描述了20个合作和基于服务的研究项目，这些项目既驱动和利用新资源。 除了对高中生，高中老师和本科生的正式课程，还将增强目前的联邦资助研究的合理生长，除了对本科生，研究生和邮政文档的培训外，还将培训培训。 除了通过谈判传播材料，我们的网站以及经过同行评审的出版物外，我们还将举办第二届国际研讨会“成像和探测单个单元格：对信号，结构和功能的应用”，以及对细胞辐射响应微动物探针的国际国际研讨会”。