Monitoring Atypical HIV Strains Among Persons Newly Dia*

监测新发感染者中的非典型艾滋病病毒毒株*

• 批准号: 7282576
• 负责人: GUTHRIE S Birkhead
• 金额: $ 16.37万
• 依托单位: HEALTH RESEARCH INC, NEW YORK STATE DOH
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-30 至 2009-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7282576
• 关键词: AIDS /HIV diagnosis; antiAIDS agent; blood tests; clinical research; cooperative study; data collection; data collection methodology /evaluation; diagnosis design /evaluation; diagnosis quality /standard; drug resistance; genetic strain; genotype; human immunodeficiency virus; human subject; method development; nucleic acid purification; patient oriented research; serology /serodiagnosis; virus genetics

DESCRIPTION (provided by applicant): Over the last two decades, AIDS reporting has formed the core of public health surveillance for HIV-related disease in the United States. More recently, all states have implemented some form of HIV reporting as well. However, the need for surveillance data on HIV incidence and atypical and antiretroviral (ARV) resistant strains has become increasingly apparent with advances in prevention, increased awareness of the international characteristics of the epidemic, and changes in the clinical course of the disease due to combination ARV therapy. Current laboratory experience would indicate that use of diagnostic sera for more expanded surveillance is feasible. An alternative to HIV confirmatory testing with sera or oral fluid that will potentially allow expanded surveillance is that of confirmatory HIV testing using dried blood spots (DBS). DBS can be prepared by individuals with minimal technical training who use a disposable lancet to create a pinpoint wound in the finger from which a few drops of blood can be absorbed onto specially prepared filter paper. The purpose of this application is to develop laboratory expertise and acquire field experience in using DBS for atypical and ARV resistant HIV strain surveillance in newly diagnosed persons. The specific aims of this application include: 1) Validate methodology for DBS atypical/ ARV resistant strain testing through paired comparison of sera and DBS; 2) Determine the feasibility and resources required to obtain DBS versus sera in clinical settings; 3) Determine the feasibility and resources required for commercial laboratories to prepare DBS from blood drawn for clinically indicated diagnostic or prognostic tests; 4) Monitor the prevalence of atypical/ARV resistant strains; and 5) Compare atypical/ARV resistant strain prevalence in sites using DBS for this surveillance versus those using sera.

描述（由申请人提供）： 在过去的二十年里，艾滋病报告已经成为美国艾滋病毒相关疾病公共卫生监测的核心。 最近，所有州也实施了某种形式的艾滋病毒报告。 然而，随着预防工作的进展、对这一流行病的国际特征的认识的提高以及由于抗逆转录病毒联合治疗而导致的疾病临床病程的变化，越来越明显地需要关于艾滋病毒发病率和非典型和抗逆转录病毒耐药菌株的监测数据。 目前的实验室经验表明，使用诊断血清进行更广泛的监测是可行的。 用血清或口腔液进行HIV确证性检测的一种替代方法是使用干血斑（DBS）进行HIV确证性检测，这可能会扩大监测范围。 DBS可以由具有最低限度技术培训的个人制备，他们使用一次性刺血针在手指上创建一个针尖伤口，从中可以将几滴血吸收到专门制备的滤纸上。 这项申请的目的是发展实验室专业知识，并获得在新诊断人员中使用DBS进行非典型和抗逆转录病毒耐药艾滋病毒毒株监测的实地经验。 本申请的具体目的包括：1）通过血清和DBS的配对比较，确定DBS非典型/ ARV耐药菌株检测的方法学; 2）确定在临床环境中获得DBS与血清的可行性和所需资源; 3）确定商业实验室从临床诊断或预后检测抽取的血液中制备DBS的可行性和所需资源; 4）监测非典型/ARV耐药菌株的流行率; 5）比较使用DBS进行监测的地点与使用血清的地点的非典型/ARV耐药菌株流行率。