Succinic Esters from Renewable Feedstocks (SERF)

工作包B1：1-2年(Iger，FA)乳酸及其衍生物的生产。乳酸菌将被用于生产乳酸及其衍生物。目前，利用糖类化合物生产乳酸受到生物耐受性不足的限制，从而导致中毒和低产量。新的更耐受的乳酸菌菌株已经被鉴定出来，这些菌株将与现有的和新的菌株一起进行评估。该工作包将使用新的和现有的乳酸菌菌株来优化乳酸生产。这些方法还将寻求通过利用植物残存生物量的不同级分来加强净化。工作包B2：1-2年级(Iger)。丁二酸及其衍生物的生产。瘤胃微生物在动物营养学中得到了广泛的研究，但尚未在拟议的工业应用背景下进行研究，见附件B技术障碍。Iger将为新应用开发这一现有的技术诀窍。几种瘤胃微生物产生琥珀酸作为主要发酵产物。琥珀酸纤维杆菌和黄色瘤胃球菌的鸡尾酒将用于生产琥珀酸，并优化发酵。工作包B3年1-2年(Iger)糖基化合物的生产。在这项工作中，将使用细胞壁降解酶，例如新型纤维素酶混合物、内切木聚糖酶、阿魏酸酯酶对未加工、机械分离和果汁/纤维组分进行处理，以确定转化效率与加工水平的关系。这项评估将通过确定最大产糖量的最低加工水平来提高经济性。此步骤将与工作包B1和B2集成。开发阶段C：平台化学品向化学原料的化学转化。在工作包B1、B2和B3中开发的平台分子将使用一系列传统和绿色合成路线转化为化学原料。在生物复合材料中心的一个新的50升设施试生产之前，将使用反应热计对该过程进行评估。然后，工业合作伙伴将验证特定应用(树脂、涂料、塑料和化妆品)工作包C1第2-3年(BC)中的产品。实验室规模的目标分子生产。将进行实验室研究(RC1)，以评估平台化学品转化为化学原料的情况，如将乳酸转化为乳酸乙酯和丙烯酸。丁二酸转化为丁二酸酯、四氢呋喃、1，4丁二醇和戊二醇的其他反应也将被研究。C2年工作包2-3年。试点规模化生产和产品配方。在适当情况下，联合体伙伴将利用工作包中制定的议定书进行化学品原料的试点规模生产。合作伙伴生产的化合物将包括聚乳酸、多元醇、多元醇酯、琥珀酸酯。

Work Package B1: Year 1-2 (IGER, FA) Production of lactic acid and its derivatives. Lactobacilli will be studied for the production of lactic acid and its derivatives. Currently lactic acid production from sugar compounds is limited by the lack of bio-tolerance which results in poisoning and low yields. New more tolerant strains of lactobacillus have been identified and these will be assessed, alongside existing and new isolates. The work package will optimise lactic acid production using novel and existing strains of lactobacilli. Methods will also seek to enhance purification by utilising different fractions from residual plant biomass. Work Package B2: Year 1-2 (IGER). Production of succinic acid and its derivatives. Rumen microbes have been studied extensively in animal nutrition but have yet to be studied in this proposed context for industrial applications, see annex B technology barriers. IGER will develop this existing know-how for new applications. Several rumen microbes produce succinate as a primary fermentation product. A cocktail of Fibrobacter succinogenes and Ruminococcus flavefaciens will be used for the production of succinic acid and optimised for fermentation. Work Package B3 Year 1-2 (IGER) Production of sugar based compounds. In this work package the use of cell wall degrading enzymes e.g. novel cellulase mixtures, endo xylanases, ferulic acid esterase on unprocessed, mechanically separated and juice/ fibre fractions will be undertaken to identify conversion efficiency vs. level of processing. This assessment will improve the economics by identifying the minimal level of processing for maximal yield of sugar. This step will be integrated with work packages B1 and B2. Development Phase C: Chemical conversions of platform chemicals to chemical feedstocks. Platform molecules developed in work packages B1, B2 and B3 will be converted into chemical feedstocks using a range of conventional and green synthesis routes. Work will assess the process using reaction calorimetry before pilot scale production on a new 50 litre facility at the BioComposites Centre. Industrial partners will then validate products in selected applications (resins, coatings, plastics and cosmetics) Work Package C1 Year 2-3 (BC). Lab scale production of target molecules. Laboratory studies (RC1) will be undertaken to assess the conversion of platform chemicals to chemical feedstocks such as lactic acid to ethyl lactate and acrylic acid. Other conversions of succinic acid to succinic esters, tetrahydrofuran, 1,4 butanediol and pentanediol will also be studied. Work Package C2 Year 2-3. Pilot scale production and product formulation. Where appropriate consortium partners will undertake pilot scale production of chemical feedstocks using the protocols developed in the work packages. Compounds made by the partners will include PLA, polyols, polyol esters, succinate esters.