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High Precision Bioassay Using Robotics and Statistics

High Precision Bioassay Using Robotics and Statistics
使用机器人和统计学进行高精度生物测定
批准号:
7613835
负责人:
David Matthew Lansky
金额:
$2.81万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2005
资助国家:
美国
项目状态:
已结题
起止时间:
2005-02-15 至 2012-02-28

项目摘要

项目成果

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中文摘要
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英文摘要
Project Summary Abstract Cell culture bioassays are often laborious and imprecise even after substantial development efforts. These bioassays are used broadly in the biotechnology industry to measure protein or vaccine products in development and for lot release. Imprecise assays and slow assay development contribute to slow product development. Common practice in cell culture bioassay ignores statistical and regulatory guidance by failing to utilize proper randomization and not accounting for group effects (i.e.; as caused by multi-channel pipettes). In addition, lack of attention to location effects within the assays, combined with simplistic analyses of assay data, compromise the precision and efficiency of cell culture bioassays. Lansky Consulting LLC will develop a standardized approach to cell culture bioassay using laboratory robots followed by modern statistical analysis. This combination will address the need for randomization, location effects, serial dilution, grouped dilution, and multiple sources of variation in assay response. The work described in this proposal will demonstrate the performance gains associated with: 1) standardized designs (including designs that require robots for randomization), 2) linear and nonlinear mixed split- or strip-plot models for bioassay analysis, and 3) advances in implementation of this modern standardized approach to bioassays. The precision gains from the combined package of modern bioassay methods will be shown to reduce the need for sample replication by an order of magnitude or more. Both simulated data and long-term lab experiments with bioassays will be used. The benefits of these new methods include lower assay costs, rapid application to new assay systems, and easy access to best practices in design, implementation, and analysis. Most importantly, by building a turnkey robotic and analysis bioassay system, this level of assay performance will be made broadly available to the biotechnology industry as well as to non-profit researchers.
期刊论文(1)
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会议论文
DOI: 10.1089/jir.2008.0043
发表时间: 2009
期刊: Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research
影响因子: --
作者: [Grossberg,SidneyE, Kawade,Yoshimi, Grossberg,LeslieD]
通讯作者: Grossberg,LeslieD
Better bioassays via designs for robots analyses with improved model selection and similarity bounds that limit potency bias
  • 批准号:
    10155988
  • 项目类别:
  • 资助金额:
    $25.2万
  • 财政年份:
    2021
  • 负责人:
    David Matthew Lansky
  • 依托单位:
High Precision Bioassay Using Robotics and Statistics
  • 批准号:
    7221731
  • 项目类别:
  • 资助金额:
    $35.47万
  • 财政年份:
    2005
  • 负责人:
    David Matthew Lansky
  • 依托单位:
High Precision Bioassay Using Robotics and Statistics
  • 批准号:
    6882967
  • 项目类别:
  • 资助金额:
    $9.98万
  • 财政年份:
    2005
  • 负责人:
    David Matthew Lansky
  • 依托单位:
High Precision Bioassay Using Robotics and Statistics
  • 批准号:
    7371144
  • 项目类别:
  • 资助金额:
    $26.07万
  • 财政年份:
    2005
  • 负责人:
    David Matthew Lansky
  • 依托单位:
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