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Core--Cell Analysis and Proteomics Core

核心--细胞分析与蛋白质组学核心

基本信息

批准号：
7663999
负责人：
RYUJI HIGASHIKUBO
金额：
$ 14.3万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7663999
关键词：
7-hydroxycoumarin Acetonitriles Acids Acridine Orange Address Adopted Air Algorithms Amino Acid Sequence Amino Acids Amplifiers Antibodies Apoptosis Area Argon Arts Aspirate substance Ataxia-Telangiectasia-Mutated protein kinase Autoradiography Back Bathing Beds Binding Biological Assay Biology Biotechnology Blast Cell Blood capillaries Boxing Bromodeoxyuridine CMEDA CMFDA Caliber Carbon Dioxide Cell Cycle Cell Fraction Cell Nucleus Cell Separation Cells Cellular Stress Chaperonin 60 Chimeric Proteins Chromatography Chromosome abnormality Collaborations Color Comet Assay Complex Computer Simulation Computer software Condition Consultations Coomassie brilliant blue R Coupled Cultured Cells Cytometry DAPI DNA DNA Damage Data Data Analyses Data Files Data Set Databases Densitometry Deposition Diagnostic Equipment Digestion Dissociation Division of Cancer Biology Drops Drug Interactions Education Educational process of instructing Electronics Electrophoretic Mobility Shift Assay Electrospray Ionization Elements Ensure Environment Excision Exposure to Expressed Sequence Tags Fever Figs - dietary Fingerprint Flow Cytometry Fluorescein-5-isothiocyanate Fluorescence Fluorescence Resonance Energy Transfer Fluorescent Dyes Fluorescent in Situ Hybridization Formic Acids Gases Gel Gene Expression Generations Glutathione S-Transferase Goals Grant Green Fluorescent Proteins HOE 33342 Heat-Shock Response Heating Hela Cells High Pressure Liquid Chromatography Homologous Gene Human Human Genome Humidity Hypoxia Image Image Analysis Implant In Situ In Situ Nick-End Labeling Incubators Individual Induction of Apoptosis Information Systems Investigation Ionizing radiation Ions Keratin Label Laboratories Lasers Life Light Liquid substance Location Maintenance Manuals Masks Mass Spectrum Analysis Measurement Measures Messenger RNA Methodology Methods Microscope Mitotic Modality Molecular Molecular Weight Monitor Mus Northern Blotting Nuclear Nuclear Matrix Nuclear Matrix-Associated Proteins Nuclear Protein Nuclear Proteins Olives - dietary Operative Surgical Procedures Optics Output Oxidation-Reduction Oxygen Pathway interactions Peptide Sequence Determination Peptide antibodies Peptides Personal Satisfaction Phase Phosphotransferases Physiologic pulse Plicamycin Ploidies Population Population Distributions Preparation Procedures Process Propidium Diiodide Protein Analysis Protein Biosynthesis Protein Chemistry Protein Databases Protein Disulfide Isomerase Proteins Proteome Proteomics Pulse taking Purpose Qualifier RNA Radiation Radiosensitization Rate Recruitment Activity Reduced Glutathione Reporting Reproducibility Research Research Personnel Resources Robot Robotics Role Route Running Sampling Scanning Science Score Screening procedure Services Silicon Dioxide Silver Silver Staining Solutions Sorting - Cell Movement Source Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Speed Spottings Staging Staining method Stainless Steel Stains Stimulus Stream Stress Superhelical DNA Support of Research Suspension substance Suspensions SwissProt System Systems Analysis Techniques Technology Temperature Time Transfection Translating Trypsin Tube Two-Dimensional Gel Electrophoresis Two-Dimensional Polyacrylamide Gel Electrophoresis Umbelliferones Universities Update Visual Washington Water Western Blotting annexin A5 assay development base calreticulin capillary cell growth charge coupled device camera computerized data processing coomassie Brilliant Blue cost effective cross reactivity crosslink data acquisition data mining design detector digital imaging evaporation experience foot formic acid gel electrophoresis human H2AX protein hyperthermia treatment instrument instrumentation ionization link protein liquid chromatography mass spectrometry macromolecule mass spectrometer molecular dynamics monochlorobimane nano neoplastic cell nucleophosmin particle photomultiplier programs promoter protein protein interaction replication factor A research study response solid state telomere thermal stress tissue culture tool transcription factor tumor two-dimensional

项目摘要

The Core Component will provide efficient, high quality support on cytometry, quantitative biology and proteomics to the Program Project. The Core will oversee the operation and/or maintenance of flow cytometry, image analysis and gel densitometry facilities, as well as the "Precision Thermal and Gas Environment Suite". Core B will also function as a connecting point for the Program Project with the Protein Chemistry Laboratory of Washington University for protein identification. The Core will also collaborate with Project researchers in developing and adopting research applications in these areas covered by the Core for their study. By consolidating these functions into the Core, we expect efficient, cost effective use of the Core resources that will benefit each Project.

核心部分将为项目项目提供高效、高质量的细胞计数、定量生物学和蛋白质组学支持。该核心将监督流式细胞术、图像分析和凝胶密度测定设施以及“精密热和气体环境套件”的操作和/或维护。核心 B 还将作为该计划项目与华盛顿大学蛋白质化学实验室的连接点鉴别。核心还将与项目研究人员合作，开发和采用核心所涵盖的这些领域的研究应用程序以进行研究。通过将这些功能整合到核心中，我们期望高效、具有成本效益地使用核心资源，这将使每个项目受益。