BD FACSARIA SYSTEM - FLOW CYTOMETER

BD FACSARIA 系统 - 流式细胞仪

• 批准号: 7216570
• 负责人: RYUJI HIGASHIKUBO
• 金额: $ 39.5万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7216570
• 关键词: Biological Assay; Biology; Cell Separation; Cells; Detection; Equipment; Flow Cytometry; Fluorescence; Fluorescent Probes; Fluorochrome; Funding; Future; Ionizing radiation; Laboratories; Lasers; Minor; Molecular; Molecular Biology; Pliability; Radiation Oncology; Research; Research Personnel; Site; Source; System; Technology; Universities; Washington; desire; medical schools; research study; tissue culture

Description (provided by applicant): Biology research in radiation oncology has extensively utilized flow cytometric analyses over the years. We, at the Radiation Oncology Department of Washington University, are no exception. We have our first flow cytometer installed in 1978. We have since conducted numerous studies using flow cytometry, with minor improvements on the equipment applied during the ensuing years. With emerging emphasis in the application of molecular biology in radiation oncology research and vast advancement in fluorescent probe technology, however, the system currently available at this site has become outdated and insufficient to conduct desired experiments. The objective of this proposal is to request funds for the procurement of a flow cytometer/sorter to replace the currently available system at the Department of Radiation Oncology at Washington University School of Medicine. The system we are requesting funds for is FACSAria manufactured by BD Biosciences. The system as requested has all the features that are required to conduct research proposed by the current and future researchers in the Department as well as investigators from other departments who has been using our facility. Having three excitation wavelength and the capability to detect up to 9 fluorescence wavelengths allow us to conduct experiments in which relationship of several molecular and cellular parameters in each cell can be simultaneously assayed. Additionally the configuration of laser excitation and fluorescence detection for this system requires lower power for fluorochrome excitation, resulting in less damage to cells that are sorted and subsequently subcultured or assayed for molecular biology parameters that are sensitive to such insults. The Radiation Oncology biology laboratory is remotely located from the main campus of the Medical School. It is the only flow cytometry facility on the Medical School campus that has immediate access to ionizing radiation sources, a major component in our research. With the availability of wet laboratory and tissue culture facility also adjacent to the flow cytometry facility, this new flow cytometer offers flexibility in conducting experiments that cannot be obtained from other flow cytometry laboratories on the Medical School campus.

描述（由申请人提供）：多年来，放射肿瘤学的生物学研究已广泛使用流式细胞术分析。我们华盛顿大学放射肿瘤学系也不例外。我们在1978年安装了第一台流式细胞仪。此后，我们使用流式细胞术进行了许多研究，并在随后的几年中对应用的设备进行了微小的改进。然而，随着放射肿瘤学研究中分子生物学应用的日益重视和荧光探针技术的巨大进步，该地点目前可用的系统已经过时，不足以进行所需的实验。本提案的目的是申请资金，用于采购一台流式细胞仪/分选机，以取代华盛顿大学医学院放射肿瘤学系现有的系统。我们申请资金的系统是由BD Biosciences制造的FACSAria。所要求的系统具有进行本部门当前和未来研究人员以及使用我们设施的其他部门调查人员提出的研究所需的所有功能。具有三个激发波长和检测多达9个荧光波长的能力使我们能够进行实验，其中可以同时测定每个细胞中几个分子和细胞参数的关系。此外，该系统的激光激发和荧光检测的配置需要较低的荧光染料激发功率，导致对细胞的损伤较小，所述细胞被分选并随后传代培养或测定对这种损伤敏感的分子生物学参数。放射肿瘤学生物实验室位于医学院主校区的远处。它是医学院校园内唯一的流式细胞仪设施，可以直接获得电离辐射源，这是我们研究的主要组成部分。随着湿实验室和组织培养设施也毗邻流式细胞仪设施的可用性，这种新的流式细胞仪在进行实验时提供了灵活性，无法从医学院校园内的其他流式细胞仪实验室获得。