Comparative Genomics in the Enterobateriaceae
肠杆菌科的比较基因组学
基本信息
- 批准号:7424031
- 负责人:
- 金额:$ 68.28万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2002
- 资助国家:美国
- 起止时间:2002-09-01 至 2010-08-31
- 项目状态:已结题
- 来源:
- 关键词:AgricultureAnimal ModelAnimalsBacteriaBase SequenceBlood specimenCaviaChickensClinicalCommunitiesConditionDataDepositionDepthDirect CostsDistressEducational process of instructingEnterobacteriaceaeErwiniaEscherichia coliEventEvolutionFamilyFamily suidaeFecesFundingFutureGenbankGenesGenomeGenomicsGoalsGrowthHigh temperature of physical objectHorizontal Gene TransferHumanHybridization ArrayHybridsIn VitroIndividualInfectionLaboratoriesLengthLibrariesManualsMedical ResearchMethodsModelingMonitorMusMutagenesisNumbersOryctolagus cuniculusPathogenicityPhenotypePhylogenetic AnalysisPlaguePlantsPoliciesPrevalenceProductionProliferatingProphagesPseudogenesRangeReadingResearch PersonnelResistanceResolutionResourcesRunningSalmonellaSalmonella entericaSalmonella infectionsSamplingShotgunsSpleenStagingStarvationStructureSurfaceSurveysSus scrofaTechnologyTestingTimeTurtlesVariantVertebral columnVirulenceVirulentWaterWeekYersinia pestisbasecomparativecostdayfood consumptionfood preparationfoodborne pathogengenome sequencingimprovedinterestmembernovelpathogenpathogenic bacteriapressureresearch studysizetherapy development
项目摘要
DESCRIPTION (provided by applicant): S. enterica subspecies I contains over 1500 different serovars with an extraordinary diversity of host ranges and pathogenic mechanisms. This continuing project will generate a resource of sequenced genomes and corresponding phenotypic data of judiciously chosen S. enterica serovars to capture some of this diversity. In the previous funding period, reductions in sequencing costs allowed us to completely sequence three Enterobacterial genomes instead of the two originally proposed. A recent leap in sequencing technology will allow the near complete sequencing of an additional 25 S. enterica genomes at the same cost. Strains will be picked for both their clinical and their taxonomic importance. Previous project results taught us that Salmonella subspecies I serovars typically share a backbone of about 90% of their -4500 chromosomal genes. The remaining 10% of each genome in these 25 strains may contain an additional 4000 new or considerably divergent genes, including prophage, some of which contain multiple embedded virulence genes. The genome sequences will permit unprecedented resolution in the study of accelerated evolution of surface markers, such as lipolysaccharide and fimbrial genes, and lateral gene transfer events. The project will also acquire data on host-range, virulence, and in vitro phenotypes for 96 strains, including each sequenced strain, to identify laboratory models to study these strains. Sequence, annotation, and phenotypic data from a large number of S. enterica strains will be made publicly available and will lay the groundwork to allow the study of mechanisms responsible for host range, pathogenic mechanism, the emergence of new variants, and will provide information that can be exploited for novel therapy development.
描述(申请人提供):肠链球菌亚种I包含1500多个不同的血清型,宿主范围和致病机制具有非凡的多样性。这个持续进行的项目将产生一个测序的基因组资源和明智地选择的肠杆菌血清型的相应表型数据,以捕捉其中的一些多样性。在之前的资助期间,测序成本的降低使我们能够完成三个肠杆菌基因组的测序,而不是最初提出的两个。最近在测序技术上的一次飞跃将允许以相同的成本对另外25个肠杆菌基因组进行接近完整的测序。将根据它们的临床和分类学重要性来选择菌株。以前的项目结果告诉我们,沙门氏菌I亚种血清型通常共享约90%的-4500染色体基因的骨架。在这25个菌株中,每个基因组的剩余10%可能包含另外4000个新的或相当不同的基因,包括原噬菌体,其中一些包含多个嵌入的毒力基因。基因组序列将在表面标记加速进化的研究中实现前所未有的分辨率,例如脂多糖和菌毛基因,以及横向基因转移事件。该项目还将获得96个菌株的寄主范围、毒力和体外表型数据,包括每个测序菌株,以确定研究这些菌株的实验室模型。大量肠杆菌菌株的序列、注释和表型数据将公之于众,并将为研究宿主范围、致病机制、新变异的出现奠定基础,并将为新疗法的开发提供信息。
项目成果
期刊论文数量(0)
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会议论文数量(0)
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Sandra W. Clifton其他文献
Sandra W. Clifton的其他文献
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{{ truncateString('Sandra W. Clifton', 18)}}的其他基金
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