Comparative Genomics in the Enterobateriaceae

肠杆菌科的比较基因组学

• 批准号: 7259830
• 负责人: Sandra W. Clifton
• 金额: $ 71.6万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7259830
• 关键词: Agriculture; Animal Model; Animals; Bacteria; Base Sequence; Blood specimen; Cavia; Chickens; Clinical; Communities; Condition; Data; Deposition; Depth; Direct Costs; Distress; Educational process of instructing; Enterobacteriaceae; Erwinia; Escherichia coli; Event; Evolution; Family; Family suidae; Feces; Funding; Future; Genbank; Genes; Genome; Genomics; Goals; Growth; High temperature of physical object; Horizontal Gene Transfer; Human; Hybridization Array; Hybrids; In Vitro; Individual; Infection; Laboratories; Length; Libraries; Manuals; Medical Research; Methods; Modeling; Monitor; Mus; Mutagenesis; Numbers; Oryctolagus cuniculus; Pathogenicity; Phenotype; Phylogenetic Analysis; Plague; Plants; Policies; Prevalence; Production; Proliferating; Prophages; Pseudogenes; Range; Reading; Research Personnel; Resistance; Resolution; Resources; Running; Salmonella; Salmonella enterica; Salmonella infections; Sampling; Shotguns; Spleen; Staging; Starvation; Structure; Surface; Surveys; Sus scrofa; Technology; Testing; Time; Turtles; Variant; Vertebral column; Virulence; Virulent; Water; Week; Yersinia pestis; base; comparative; cost; day; food consumption; food preparation; foodborne pathogen; genome sequencing; improved; interest; member; novel; pathogen; pathogenic bacteria; pressure; research study; size; therapy development

DESCRIPTION (provided by applicant): S. enterica subspecies I contains over 1500 different serovars with an extraordinary diversity of host ranges and pathogenic mechanisms. This continuing project will generate a resource of sequenced genomes and corresponding phenotypic data of judiciously chosen S. enterica serovars to capture some of this diversity. In the previous funding period, reductions in sequencing costs allowed us to completely sequence three Enterobacterial genomes instead of the two originally proposed. A recent leap in sequencing technology will allow the near complete sequencing of an additional 25 S. enterica genomes at the same cost. Strains will be picked for both their clinical and their taxonomic importance. Previous project results taught us that Salmonella subspecies I serovars typically share a backbone of about 90% of their -4500 chromosomal genes. The remaining 10% of each genome in these 25 strains may contain an additional 4000 new or considerably divergent genes, including prophage, some of which contain multiple embedded virulence genes. The genome sequences will permit unprecedented resolution in the study of accelerated evolution of surface markers, such as lipolysaccharide and fimbrial genes, and lateral gene transfer events. The project will also acquire data on host-range, virulence, and in vitro phenotypes for 96 strains, including each sequenced strain, to identify laboratory models to study these strains. Sequence, annotation, and phenotypic data from a large number of S. enterica strains will be made publicly available and will lay the groundwork to allow the study of mechanisms responsible for host range, pathogenic mechanism, the emergence of new variants, and will provide information that can be exploited for novel therapy development.

描述（由申请人提供）：肠链球菌I亚种包含超过1500种不同的血清型，具有宿主范围和致病机制的非凡多样性。这个持续的项目将产生一个基因组测序资源和相应的肠链球菌血清型的表型数据，以捕获这种多样性。在之前的资助期内，测序成本的降低使我们能够完成三个肠杆菌基因组的测序，而不是最初提出的两个。最近测序技术的飞跃将允许以相同的成本对另外25个肠球菌基因组进行近乎完整的测序。菌株将根据其临床和分类学重要性来选择。先前的项目结果告诉我们，沙门氏菌I亚型血清型通常共享约90%的-4500染色体基因的主干。在这25个菌株中，每个基因组的剩余10%可能包含额外的4000个新的或相当不同的基因，包括前噬菌体，其中一些含有多个嵌入的毒力基因。基因组序列将允许前所未有的分辨率在加速进化的表面标记，如脂多糖和毛状基因，和横向基因转移事件的研究。该项目还将获取96个菌株（包括每个测序菌株）的宿主范围、毒力和体外表型数据，以确定研究这些菌株的实验室模型。大量肠链球菌菌株的序列、注释和表型数据将被公开，这将为研究宿主范围、致病机制、新变异的出现机制奠定基础，并将为开发新的治疗方法提供信息。