Qualification of Novel Immune Markers in Human Serum of Patients with MS

多发性硬化症患者人血清中新型免疫标志物的鉴定

• 批准号: 7615219
• 负责人: Michelle Sexton
• 金额: $ 4.58万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-30 至 2011-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7615219
• 关键词: Acute; Autoimmune Diseases; Calculi; Cannabinoids; Central Nervous System Diseases; Class; Clinical Research; Complementary and alternative medicine; Condition; Data; Disease; Doctor of Philosophy; Eicosanoids; Endocannabinoids; Essential Fatty Acids; Fellowship; Fish Oils; Funding; Gastrointestinal Diseases; Glaucoma; Goals; Healed; Health; Health Professional; Human; Immune; Immunologic Markers; Inflammation; Inflammatory; Intervention; Leukocytes; Measures; Mediating; Membrane; Mentors; Mission; Molecular Mechanisms of Action; Multiple Sclerosis; National Center for Complementary and Alternative Medicine; Naturopathic Doctor; Neurodegenerative Disorders; Osteoporosis; Patients; Peripheral; Pharmacology; Phospholipids; Placebo Control; Placebos; Production; Public Health; Randomized; Rate; Relapse; Research; Research Personnel; Science; Serum; Signal Transduction; Supplementation; System; Therapeutic; Time; Training; Training Support; Universities; Unsaturated Fatty Acids; Washington; base; cell motility; chronic pain; cost; essential phospholipids; healing; human migration; improved; interest; lipid biosynthesis; nervous system disorder; novel; outcome forecast; receptor; receptor expression

DESCRIPTION (provided by applicant): The long-term objective of this fellowship proposal is to train Michelle Sexton, a Naturopathic Doctor (candidate June 2008), to become an independent researcher. In this mentored research plan, she will investigate the effects of fish oil supplementation, a common Complementary Alternative Medicine (CAM) therapy, on newly recognized markers of inflammation, namely the components forming the endocannabinoid (eCB) system. In order to achieve this goal, and as a stepping-stone into interdisciplinary mechanistic studies, the candidate will be sponsored by Nephi Stella PhD in the Pharmacology Department of the University of Washington. The primary aims of this research are: Aim 1: To measure endocannabinoids (eCBs) in human serum to determine if there is a difference between healthy subjects and patients with Multiple Sclerosis (MS). We will also evaluate whether supplementation with fish oil restores these levels. Aim 2: To measure Cannabinoid 2 (CB2) receptor expression in human leukocytes. Aim 3: To measure CB2 receptor-mediated migration by human leukocytes. This study will also for the first time quantify eCBs in the periphery of patients with MS. This is significant for public health because the ability to modulate the functionality of the eCB signaling system by supplementing fish oil could provide low-cost, safe therapeutic benefit to patients with MS. This is relevant because there are approximately 300,000 patients in the US alone with MS. In addition, patients suffering from acute and chronic pain, autoimmune disease, gastrointestinal disorders, glaucoma, osteoporosis and a host of central nervous system disorders could also benefit from fish oil supplementation. All of these are conditions that have been shown to have an association with the eCB system. Funding of this proposal will be in accord with the missions of NCCAM which are: * Explore complementary and alternative healing practices in the context of rigorous science. * Train complementary and alternative medicine researchers. * Disseminate authoritative information to the public and professionals. This F32 will provide support for the training of a new investigator involved in research which will help the public and health professional understand more about mechanisms of action of a CAM therapy.

描述（由申请人提供）：本奖学金提案的长期目标是培训米歇尔塞克斯顿，自然疗法医生（候选人2008年6月），成为一个独立的研究人员。在这项指导研究计划中，她将研究鱼油补充剂（一种常见的补充替代医学（CAM）疗法）对新识别的炎症标志物（即形成内源性大麻素（eCB）系统的成分）的影响。为了实现这一目标，并作为跨学科机制研究的垫脚石，候选人将由华盛顿大学药理学系的Nephi Stella博士赞助。 本研究的主要目的是： 目标1：测量人血清中的内源性大麻素（eCB），以确定健康受试者和多发性硬化症（MS）患者之间是否存在差异。我们还将评估补充鱼油是否能恢复这些水平。 目的2：检测大麻素2（CB 2）受体在人白细胞中的表达。 目的3：检测CB 2受体介导的人白细胞迁移。 这项研究还将首次量化MS患者外周中的eCB。这对公共卫生具有重要意义，因为通过补充鱼油调节eCB信号系统功能的能力可以为MS患者提供低成本，安全的治疗益处。这是相关的，因为仅在美国就有大约30万MS患者。患有急性和慢性疼痛、自身免疫性疾病、胃肠道疾病、青光眼、骨质疏松症和许多中枢神经系统疾病的患者也可以从鱼油补充剂中受益。所有这些都是已被证明与eCB系统相关的条件。本提案的资金筹措将与国家CAM委员会的任务雅阁，这些任务是： * 在严格的科学背景下探索补充和替代治疗实践。 * 培训补充和替代医学研究人员。 * 向公众和专业人士传播权威信息。 该F32将为参与研究的新研究者的培训提供支持，这将有助于公众和卫生专业人员更多地了解CAM治疗的作用机制。