Cell migration: The roles of Prostaglandins

细胞迁移：前列腺素的作用

• 批准号: 7487522
• 负责人: Tina L Tootle
• 金额: $ 4.84万
• 依托单位: CARNEGIE INSTITUTION OF WASHINGTON, D.C.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7487522
• 关键词: Alleles; Animal Model; Biogenesis; Biological; Biological Models; Cell Adhesion Molecules; Defect; Drosophila genus; Event; Fatty Acids; Genes; Genetic; Genetic Screening; Human; Immune; Immunity; Inflammation; Malignant Neoplasms; Mediating; Methods; Molecular; Mutation; Neoplasm Metastasis; Normal Cell; Oogenesis; Phenotype; Process; Prostaglandins; Reaction; Reproduction; Role; Signal Pathway; Signal Transduction; Signaling Molecule; Signaling Pathway Gene; Site; Somatic Cell; Study models; cell motility; insight; migration; mutant; novel; novel diagnostics; progesterone 11-hemisuccinate-(2-iodohistamine); prostaglandin transporter; research study; therapeutic target; tool; tumor progression

DESCRIPTION (provided by applicant): While prostaglandins (PCs), fatty acid derived signaling molecules, mediate cell migration during inflammation, immune reactions, reproduction, and cancer, the mechanisms by which PGs regulate migration remain unclear. It is likely that PGs contribute to cell migration through similar means during both normal cell migrations and those that take place during cancer metastasis. As PG synthesis and signaling components have been evolutionary conserved, one can utilize the genetic tools available in Drosophila to uncover the activities and signaling events mediated by PGs during cell migrations at a level of detail that cannot easily be obtained in other species. To establish Drosophila as a model for studying PG signaling the expression and mutant phenotypes of conserved PG synthesis and signaling components will be characterized. Then, using a genetic interaction screen the PG biogenesis genes involved in cell migration, as well as which signal transduction components and cell adhesion molecules important for PG mediated migrations will be determined. As PG synthesis is upregulated in numerous cancers, the results may reveal novel mechanisms of cancer progression and identify new diagnostic tools or therapeutic targets for cancer.

描述（由申请人提供）：前列腺素（PCs）是脂肪酸衍生的信号分子，在炎症、免疫反应、生殖和癌症过程中介导细胞迁移，但pg调节迁移的机制尚不清楚。在正常细胞迁移和癌症转移过程中，pg可能通过类似的方式促进细胞迁移。由于PG合成和信号传导成分在进化上是保守的，人们可以利用果蝇中可用的遗传工具来揭示PG在细胞迁移过程中介导的活动和信号传导事件的细节水平，这在其他物种中很难获得。为了将果蝇作为研究PG信号的模型，我们将对保守的PG合成和信号组分的表达和突变表型进行表征。然后，利用遗传互作筛选参与细胞迁移的PG生物发生基因，以及确定哪些信号转导成分和细胞粘附分子对PG介导的迁移至关重要。由于PG合成在许多癌症中上调，该结果可能揭示癌症进展的新机制，并确定新的癌症诊断工具或治疗靶点。

项目成果

Stem cells and their niches: integrated units that maintain Drosophila tissues.

• DOI: 10.1101/sqb.2008.73.023
• 发表时间: 2008
• 期刊: Cold Spring Harbor symposia on quantitative biology
• 作者: A. Spradling;Todd G. Nystul;D. Lighthouse;L. Morris;Donald T. Fox;Rachel T. Cox;Tina L. Tootle;R. Frederick;Andrew D. Skora