Ultrasonic manipulation and transport of DNA molecules in evanescent light fields

倏逝光场中 DNA 分子的超声操纵和传输

• 批准号: EP/D03454X/1
• 负责人: Martyn Hill
• 金额: $ 48.4万
• 依托单位: University of Southampton
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2006
• 资助国家: 英国
• 起止时间: 2006 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FD03454X%2F1
• 关键词: Ultrasonic; manipulation; transport; DNA; molecules

Ultrasonic Standing Waves (USWs) can be used to manipulate particles within a fluid. Used within chambers of a half wavelength depth or less, the ultrasonic radiation forces can move the particles either to a central plane within the chamber or to a wall of the chamber. This project is a multidisciplinary investigation into the use of USWs in microfluidic biosensing applications. It involves the School of Engineering Sciences (SES), the Optoelectronics Research Centre (ORC) and the Microelectronics Research Centre in the Department of Electronics and Computer Science (ECS) at the University of Southampton. The work aims to integrate optical sensing and USW excitation using techniques and materials compatible with emerging microfluidic technology. It will also use the optical/USW techniques to study and quantify the behaviour of the USW chamber, particularly with regard to the ability of quarter wavelength chambers to move particles to the chamber wall. The project will apply these techniques to the detection of a number of DNA sequences in a mixture. It will also investigate the feasibility of using acoustic radiation forces to interrogate single DNA sequences with a high resolution.

超声驻波（USW）可用于操纵流体中的颗粒。在半波长深度或更小的腔室内使用，超声辐射力可以将颗粒移动到腔室内的中心平面或腔室的壁。该项目是对超声波在微流体生物传感应用中的使用进行的多学科研究。它涉及工程科学学院（SES），光电研究中心（ORC）和电子和计算机科学系（ECS）的微电子研究中心在南安普顿大学。这项工作的目的是使用与新兴微流体技术兼容的技术和材料来集成光学传感和USW激发。它还将使用光学/USW技术来研究和量化USW腔室的行为，特别是关于四分之一波长腔室将颗粒移动到腔室壁的能力。该项目将应用这些技术来检测混合物中的一些DNA序列。它还将研究使用声辐射力以高分辨率询问单个DNA序列的可行性。

项目成果

Multi-modal particle manipulator to enhance bead-based bioassays

多模式粒子操纵器可增强基于珠子的生物测定

• DOI: 10.1016/j.phpro.2010.01.036
• 发表时间: 2010
• 期刊: Physics Procedia
• 作者: Glynne-Jones P