Copolymerized Micelles as Pseuodophases in Micellar Electrokinetic Chromatography

胶束电动色谱中作为伪相的共聚胶束

• 批准号: 7491080
• 负责人: CEVDET AKBAY
• 金额: $ 13.21万
• 依托单位: FAYETTEVILLE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7491080
• 关键词: Amino Acids; Bachelor' s Degree; Capillary Electrophoresis; Carbon; Chromatography; Class; Disadvantaged; Educational Activities; Equilibrium; Fluorescence; Gas Chromatography; Goals; Head; Inorganic Sulfates; Laboratory Research; Lead; Leucine; Link; Liquid Chromatography; Mentors; Micelles; Modeling; Molecular; Monitor; Nadolol; Nuclear Magnetic Resonance; Numbers; Optics; Pharmaceutical Preparations; Phase; Process; Range; Reaction; Recruitment Activity; Research; Research Activity; Research Personnel; Sample Size; Science; Series; Spectroscopy, Fourier Transform Infrared; Students; System; Tail; Techniques; Time; Training; Universities; Unspecified or Sulfate Ion Sulfates; analytical method; base; carboxylate; career; chiral molecule; cost; enantiomer; experience; insight; interest; migration; monomer; novel; physical property; programs; surfactant; tool

The use of CE for analytical separations has many advantages over the other two commonly used chromatographic techniques, that is, gas chromatography and liquid chromatography. Some of these advantages include lower operating costs, much higher separation efficiency, small sample size required, short analysis time, versatility, and simplicity. Micellar electrokinetic chromatography (MEKC) was introduced in 1984 and is the CE mode most commonly used for enantiomeric separation. While MEKC is a very useful technique, it has several disadvantages. One disadvantage is that in order for the surfactant to be effective as a pseudostationary phase, it must be at a concentration above its CMC. In addition, the dynamic equilibrium between the free surfactant molecules and the micelle can lead to a decrease in separation efficiency as compared to a more ordered micellar system. To overcome these drawbacks, polymeric surfactants (or molecular micelles) were introduced. First advantage of molecular micelles is the elimination of the dynamic equilibrium between monomer and the micelle. Second advantage is the lack of a CMC, thus they may be used at concentrations well below the CMC of the unpolymerized surfactants. Single amino acid-based polymeric surfactants as chiral selectors for MEKC were first introduced in 1994. A major advantage of this approach is that different functionalities, such as a variety of chiral head groups (e.g., amino acids), can be integrated into the polymeric surfactants. This introduces an effective way of manipulating selectivity of the pseudostationary phase. In addition, the availability of both D and L optical configurations of amino acid-based pseudostationary phases is particularly advantageous to more accurately determine enantiomeric impurities by reversal of the migration order of the two enantiomers. The main disadvantage of these types of molecular micelles is that they are not soluble below pH 7.0 due to carboxylated head groups (e.g., amino acids). The PI intends to develop a new class of amino acid based polymeric surfactants that could be used at wider pH ranges. Highly soluble sulfated achiral surfactants and amino acid basid chiral surfactants will be copolymerized at various molar ratios to form novel copolymerized molecular micelles. These surfactants will be characterized using a variety of analytical methods and will be applied as pseudostationary phases in MEKC for separation of chiral molecules at a wider pH range.

使用毛细管电泳进行分析分离比其他两种常用的方法有许多优点 色谱技术，即气相色谱和液相色谱。其中一些 优点包括较低的操作成本，高得多的分离效率，所需的小样品尺寸， 分析时间短、多功能性和简单性。介绍了胶束电动色谱 在1984年，并且是最常用于对映体分离的CE模式。虽然MEKC是一个非常有用的 技术，它有几个缺点。一个缺点是，为了使表面活性剂有效， 作为假固定相，其浓度必须高于其CMC。此外，动态 自由表面活性剂分子和胶束之间的平衡可导致分离的降低 与更有序的胶束系统相比，效率更高。为了克服这些缺点，聚合物 引入表面活性剂（或分子胶束）。分子胶束的第一个优点是消除 单体和胶束之间的动态平衡。第二个优点是没有CMC，因此 它们可以在远低于未聚合的表面活性剂的CMC的浓度下使用。个单氨基酸 1994年首次引入了酸基聚合物表面活性剂作为MEKC的手性选择剂。一个主要 该方法的优点是不同的官能团，例如各种手性头基（例如， 氨基酸）可以结合到聚合物表面活性剂中。这是一种有效的方法， 操纵假固定相的选择性。此外，D和L光学 基于氨基酸的假固定相的构型特别有利于更准确地测定 通过颠倒两种对映体的迁移顺序来测定对映体杂质。主要 这些类型的分子胶束的缺点是它们在低于pH 7.0时不可溶， 羧基化头部基团（例如，氨基酸）。PI打算开发一类新的基于氨基酸的 可以在更宽的pH范围内使用的聚合物表面活性剂。高度可溶的硫酸化非手性表面活性剂和 氨基酸手性表面活性剂将以不同的摩尔比共聚，形成新型的共聚型手性表面活性剂， 分子胶束这些表面活性剂将使用各种分析方法表征，并将通过 在较宽的pH范围内作为假固定相用于手性分子的分离。