Confocal Microscopy Imaging System

共焦显微镜成像系统

• 批准号: 7220471
• 负责人: MURAD OOKHTENS
• 金额: $ 41.51万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-15 至 2008-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7220471
• 关键词: Aging; Applications Grants; Arts; Biochemistry; Biophysics; Budgets; Cells; Cellular biology; Color; Confocal Microscopy; Dentistry; Drug Delivery Systems; Drug toxicity; Equipment; Eye; Faculty; Fluorescence Recovery After Photobleaching; Fluorescence Resonance Energy Transfer; Funding; Grant; Heart Injuries; Hepatitis C; Housing; Image; Kidney; Label; Life; Liver; Liver diseases; Lung; Maintenance; Medicine; Methods; Microscope; Microscopy; Pathology; Pharmacologic Substance; Physiology; Qualifying; Research; Research Infrastructure; Research Personnel; Resources; Science; Services; Specialist; Specimen; System; Techniques; Time; Tooth structure; United States National Institutes of Health; base; experience; fluorophore; instrument; instrumentation; medical schools; novel; problem drinker; ranpirnase

DESCRIPTION (provided by applicant): This shared instrumentation grant application is to acquire a state-of-the-art confocal microscopy imaging system (Zeiss LSM 510 core unit + critical upgrades) to replace our aging, limited-capability system (Nikon PCM 2000) and allow our user base to expand into critical and novel applications/techniques. Our present user base of 19 NIH-funded investigators with qualifying grants is comprised of faculty from several departments in the Schools of Medicine (Medicine, Pathology, Physiology and Biophysics, Biochemistry), Pharmaceutical Sciences and Dentistry. They have been using our existing system housed in the Confocal Microscopy Subcore of the Cell Biology Core of USC's Research Center for Liver Diseases (RCLD). This facility has been the sole and/or primary confocal microscopy resource they have access to. Although Nikon PCM 2000 is a workhorse instrument, it is capable of only simple 2-color imaging in fixed specimens and lacks advanced capabilities, such as live cell and time-lapse imaging, imaging specimens labeled with 3 or 4 fluorophores, fluorescence recovery after photobleaching, or fluorescence resonance energy transfer, which are sorely needed by our user base. The other four existing system on our campus are either dedicated to special uses or overloaded with their own user base. The new system will replace our old and be housed-operated in the same facility, with space/darkroom in place. RCLD is funded by NIH P30 DK 48522, Dr. Neil Kaplowitz, P.I., which is in its 3rd 5-yr cycle (3/1/05-2/28/10). It has an established track record in terms of all the necessary infrastructure, administrative, technical (full-time microscopy specialist) and financial (budget for warranty service-maintenance of microscope), to maintain and operate the new equipment. Drs. Murad Ookhtens and Sarah Hamm-Alvarez, the P.I. and co-P.I. on this application, serve as co-directors of the Cell Biology Core, a subcore of which houses the confocal microscopy equipment. They are highly experienced investigators, with extensive cumulative record and expertise in overseeing the use of a large base of sophisticated instrumentation and will share in technical oversight and management of the use of the new system, as they have done with the old. Relevance: The equipment will substantially advance the research of faculty involved in studying the normal and diseased states of the liver (hepatitis C, alcoholic/drug toxicity and injury), heart, lung, kidney, eye, teeth, as well as investigating various methods of drug delivery.

描述（由申请人提供）：此共享仪器拨款申请是为了获得最先进的共焦显微成像系统（蔡司LSM 510核心单元+关键升级），以取代我们老化，功能有限的系统（尼康PCM 2000），并允许我们的用户群扩展到关键和新颖的应用/技术。我们目前的用户群19 NIH资助的研究人员与合格的赠款是由来自医学院（医学，病理学，生理学和生物物理学，生物化学），药物科学和牙科的几个部门的教师。他们一直在使用我们现有的系统，该系统位于南加州大学肝病研究中心（RCLD）细胞生物学核心的共聚焦显微镜子核心。该设施一直是唯一和/或主要的共聚焦显微镜资源，他们可以访问。虽然Nikon PCM 2000是一台主力仪器，但它只能在固定标本中进行简单的双色成像，并且缺乏先进的功能，例如活细胞和延时成像，标记有3或4个荧光团的标本成像，光漂白后的荧光恢复或荧光共振能量转移，这些都是我们的用户群迫切需要的。我们校园里现有的其他四个系统要么是专用于特殊用途，要么是因为它们自己的用户群太多。新系统将取代我们的旧系统，并在同一设施中进行室内操作，并配备空间/暗室。RCLD由NIH P30 DK 48522资助，Neil Kaplowitz博士，P.I.，这是其第三个5年周期（3/1/05-2/28/10）。它在所有必要的基础设施、行政、技术（全职显微镜专家）和财务（保修服务-显微镜维护预算）方面都有既定的记录，以维护和操作新设备。私人侦探穆拉德·欧腾斯和莎拉·哈姆·阿尔瓦雷斯博士和联合私家侦探在这个应用程序中，担任细胞生物学核心的共同主任，其中一个子核心容纳共聚焦显微镜设备。他们都是经验丰富的调查员，在监督大量精密仪器的使用方面有着广泛的累积记录和专门知识，并将像对旧系统一样，共同对新系统的使用进行技术监督和管理。相关性：该设备将大大促进参与研究肝脏（丙型肝炎，酒精/药物毒性和损伤），心脏，肺，肾脏，眼睛，牙齿的正常和疾病状态的教师的研究，以及研究各种药物输送方法。