Shared Instrumentation Grant

共享仪器补助金

• 批准号: 7216551
• 负责人: ZSOLT SZABO
• 金额: $ 27.35万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7216551
• 关键词: Algorithms; Alpha Particles; Autoradiography; Cancer Detection; Cardiovascular Diseases; Cell Proliferation Regulation; Charge; Collection; Data; Detection; Development; Devices; Diagnostic Procedure; Discrimination; Drug Kinetics; Drug or chemical Tissue Distribution; Electrons; Funding; Funding Applicant; Grant; Image; Investigation; Isotopes; Label; Ligands; Malignant Neoplasms; Measurement; Measures; Metabolism; Methodology; Methods; Molecular Target; Pharmaceutical Preparations; Positron; Positron-Emission Tomography; Proteins; Public Health; Radiation; Radionuclide therapy; Radiopharmaceuticals; Range; Research Personnel; Resolution; Specificity; System; Time; Tissues; United States National Institutes of Health; Universities; Validation; Vascularization; base; dosimetry; improved; instrument; instrumentation; particle; protein expression; radioligand; radiotracer; response; single photon emission computed tomography

DESCRIPTION (provided by applicant): Funds are requested for acquisition of a real time charged particle autoradiographic imager for the Johns Hopkins University. This device will assist molecular target detection (whole body and tissue distribution, target specificity), radioligand development (pharmacokinetics and pharmacological specificity, correlation with protein expression), radiopharmaceutical dosimetry (both whole body and tissue dosimetry). Investigations of protein regulation, cell proliferation, tissue metabolism and vascularization will also be enhanced. The Biospace Measures Beta Imager 2000Z charged particle imaging system (2000Z) has been selected because of its multiple advantages. Its large field of view will permit whole body autoradiography and its high sensitivity will permit imaging of H-3 labeled ligands. With the 2000Z positrons, betas, alphas and electrons associated with various radiotracers can be detected at spatial resolutions of 60 to 500 micrometer, at a detection threshold of 0.007 cpm/mm2 for 3H and with an efficiency as high as 90%. Linearity of the imager is within 2 % over the dynamic range of 104. The 2000Z Imager allows simultaneous detection of two or three isotopes using decay time differences or charged particle energies for discrimination. Multiple labeling opens up new applications with simultaneous quantification of several molecular targets at a time which will support drug and radioligand selectivity studies. Data are collected in list mode making it possible to retrospectively examine images acquired over different collection time-periods. The device can display the collected image in real-time, thereby eliminating under and over-exposures. RELEVANCE: The 2000Z Imager will be regularly employed by a major group of NIH funded researchers. Each of the included projects have strong NIH funding and are relevant to public health. They cover development of radiopharmaceuticals and radiation methodologies to treat cancer and diagnostic methods for improved detection of cancer and cardiovascular diseases. This instrument will have a strong impact on the development of new radioligands and for validation of new quantification algorithms for PET and SPECT. It will be also essential in the development of radionuclide therapies based on beta and alpha particle radiation and will significantly impact selection of ligands and isotopes, methods of delivery, estimation of dosimetry, and measurement of treatment response.

描述(由申请人提供)：为约翰霍普金斯大学购买实时带电粒子放射自显影成像仪申请资金。该设备将辅助分子靶标检测(全身和组织分布、靶标特异性)、放射性配基开发(药代动力学和药理学特异性、与蛋白质表达的相关性)、放射性药物剂量测定(全身和组织剂量测定)。蛋白质调节、细胞增殖、组织代谢和血管形成的研究也将得到加强。生物空间测量Beta Imager 2000Z带电粒子成像系统(2000Z)因其多重优势而被选中。它的大视野将允许全身放射自显影，其高灵敏度将允许成像H-3标记的配体。利用2000Z正电子，可以在60-500微米的空间分辨率下探测各种放射性示踪剂的β、α和电子，对~3H的探测阈值为0.007 cpm/mm~2，探测效率高达90%。在104的动态范围内，成像器的线性度在2%以内。2000Z成像仪允许使用衰变时间差或带电粒子能量进行区分，同时检测两个或三个同位素。多重标记开辟了新的应用领域，可以同时定量几个分子靶标，这将支持药物和放射性配基的选择性研究。数据是以列表模式收集的，因此可以回溯检查在不同收集时间段内获取的图像。该设备可以实时显示采集的图像，从而消除曝光不足和过度曝光。相关性：NIH资助的主要研究人员将定期使用2000Z成像仪。每个纳入的项目都有强大的NIH资金，并与公共卫生相关。它们包括开发治疗癌症的放射性药物和辐射方法，以及改进癌症和心血管疾病检测的诊断方法。该仪器将对开发新的放射性配基和验证PET和SPECT的新定量算法产生重大影响。它也将在开发基于贝塔和阿尔法粒子辐射的放射性核素疗法方面发挥重要作用，并将对配体和同位素的选择、输送方法、剂量估算和治疗反应的测量产生重大影响。