Acquisition of a Bruker E580 Pulse EPR Spectrometer

购买 Bruker E580 脉冲 EPR 光谱仪

• 批准号: 7210413
• 负责人: CANDICE S KLUG
• 金额: $ 50万
• 依托单位: MEDICAL COLLEGE OF WISCONSIN
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7210413
• 关键词: Arts; Communities; Electron Nuclear Double Resonance; Electron Spin Resonance Spectroscopy; Electrons; Environment; Funding; Funding Applicant; Measures; Medical; Metals; Methods; Minor; Peptides; Physiologic pulse; Productivity; Proteins; Pulse taking; Range; Research; Research Personnel; Running; Site; Spin Labels; System; Techniques; Temperature; Wisconsin; college; cryogenics; instrument; instrumentation; protein structure; quantum; research study

DESCRIPTION (provided by applicant): Funds are requested to purchase a Bruker ELEXSYS 580 pulse spectrometer capable of running double electron electron resonance (DEER), double quantum coherence (DQC), and electron nuclear double resonance (ENDOR) experiments at cryogenic temperatures. Pulse instrumentation of this kind is not available at the Medical College of Wisconsin (MCW), or in the surrounding regional community. Recent advances in the commercial instrumentation and application of pulsed EPR spectroscopy are rapidly making this technique essential to the site-directed spin labeling (SDSL) and metal EPR research communities. The ability of DEER and DQC methods to measure interspin distances in the range of 15-50¿ opens up an entirely new range of possibilities in the study of biomedically significant research problems. Five major users will utilize this instrument to measure long distance ranges within protein or peptide systems. In addition, numerous minor users are identified, each with a need for advanced pulse instrumentation not available in the region. Each major user is NIH-funded and has an established and growing research group dedicated to the advancement of protein structure and functional dynamics. This instrumentation will immediately and significantly advance the productivity of the projects outlined in this proposal in addition to enhancing the research environment for the surrounding community. Having the pulse capability that this instrumentation will provide is vital to the rapid progress of the discussed NIH-funded projects as well as to providing MCW researchers and visitors access to state-of-the-art EPR instrumentation.

描述（申请人提供）：申请资金用于购买Bruker ELEXPENDIX 580脉冲光谱仪，该光谱仪能够在低温下进行双电子电子共振（DEER）、双量子相干（DQC）和电子核双共振（ENDOR）实验。威斯康星州医学院（MCW）或周围的区域社区没有这种脉冲仪器。脉冲EPR光谱的商业仪器和应用的最新进展正在迅速使这项技术对定点自旋标记（SDSL）和金属EPR研究社区至关重要。DEER和DQC方法测量15-50 º范围内的自旋间距离的能力为研究生物医学重要研究问题开辟了全新的可能性范围。 五个主要用户将利用该仪器测量蛋白质或肽系统内的长距离范围。此外，还确定了许多小用户，每个用户都需要该区域没有的先进脉冲仪器。每个主要用户都是NIH资助的，并拥有一个致力于蛋白质结构和功能动力学进步的成熟和不断发展的研究小组。该仪器将立即显着提高本提案中概述的项目的生产力，并改善周围社区的研究环境。 拥有这种仪器将提供的脉冲能力对于所讨论的NIH资助项目的快速进展以及为MCW研究人员和访问者提供最先进的EPR仪器至关重要。