Acquisition of a Bruker E580 Pulse EPR Spectrometer

描述（由申请人提供）：申请资金用于购买布鲁克ELEXSYS 580脉冲光谱仪，该脉冲光谱仪能够在低温下进行双电子电子共振（DEER），双量子相干（DQC）和电子核双共振（ENDOR）实验。这种脉搏仪器在威斯康辛医学院（MCW）或周边地区社区是不可用的。商业仪器和脉冲EPR光谱应用的最新进展使该技术迅速成为定位自旋标记（SDSL）和金属EPR研究界的重要技术。DEER和DQC方法测量15-50°范围内的互旋距离的能力为生物医学重大研究问题的研究开辟了一个全新的可能性范围。

DESCRIPTION (provided by applicant): Funds are requested to purchase a Bruker ELEXSYS 580 pulse spectrometer capable of running double electron electron resonance (DEER), double quantum coherence (DQC), and electron nuclear double resonance (ENDOR) experiments at cryogenic temperatures. Pulse instrumentation of this kind is not available at the Medical College of Wisconsin (MCW), or in the surrounding regional community. Recent advances in the commercial instrumentation and application of pulsed EPR spectroscopy are rapidly making this technique essential to the site-directed spin labeling (SDSL) and metal EPR research communities. The ability of DEER and DQC methods to measure interspin distances in the range of 15-50¿ opens up an entirely new range of possibilities in the study of biomedically significant research problems. Five major users will utilize this instrument to measure long distance ranges within protein or peptide systems. In addition, numerous minor users are identified, each with a need for advanced pulse instrumentation not available in the region. Each major user is NIH-funded and has an established and growing research group dedicated to the advancement of protein structure and functional dynamics. This instrumentation will immediately and significantly advance the productivity of the projects outlined in this proposal in addition to enhancing the research environment for the surrounding community. Having the pulse capability that this instrumentation will provide is vital to the rapid progress of the discussed NIH-funded projects as well as to providing MCW researchers and visitors access to state-of-the-art EPR instrumentation.