PHOSPHOENOLPYRUVATE CARBOXYKINASE, FROM E COLI

磷酸酚丙酮酸羧激酶，来自大肠杆菌

• 批准号: 7358931
• 负责人: LOUIS DELBAERE
• 金额: $ 0.57万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7358931
• 关键词: PHOSPHOENOLPYRUVATE; CARBOXYKINASE; COLI

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. PCK plays a critical role in gluconeogenesis. Under saturating conditions of Mg and ATP, PCK is activated 6 fold by the addition of Ca and 4 fold by Mn. Digestion of PCK by trypsin results in the cleavage of 25% of the C-terminus. Trypsin digested PCK, or truncated PCK, lost its ability to be activated by Ca; however Mn activation remained. Truncated PCK was found to reattain 2-fold activation by addition of Ca. In the hope of attaining a clear understanding of Pck activation by Ca and Mn, and how Ca activation can be regained four unique truncated Pck complexes were crystallized. It is believed knowledge of the crystal structure of truncated PCK may aid in the treatment of diabetes. Specifically it is believed a PCK inhibitor may one day reduced the need for insulin.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。 PCK 在糖异生中发挥着关键作用。在 Mg 和 ATP 饱和条件下，添加 Ca 可使 PCK 活化 6 倍，添加 Mn 可活化 4 倍。胰蛋白酶消化 PCK 导致 25% 的 C 末端裂解。胰蛋白酶消化的 PCK，或截短的 PCK，失去了被 Ca 激活的能力；然而，Mn 的活化作用仍然存在。发现截短的 PCK 通过添加 Ca 可以重新获得 2 倍的激活。为了清楚地了解 Ca 和 Mn 对 Pck 的激活，以及如何重新获得 Ca 的激活，我们结晶了四种独特的截短 Pck 复合物。人们相信，了解截短的 PCK 晶体结构可能有助于治疗糖尿病。具体而言，据信 PCK 抑制剂有一天可能会减少对胰岛素的需求。