Optical Coherence Microscopy for Endoscopic Diagnostics

用于内窥镜诊断的光学相干显微镜

• 批准号: 7354851
• 负责人: Aaron D Aguirre
• 金额: $ 1.18万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-01-16 至 2008-04-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7354851
• 关键词: Animal Model; Animals; Area; Biopsy Specimen; Boston; California; Cancerous; Clinical; Clinical Research; Clinical Trials; Collaborations; Colon; Confocal Microscopy; Cytoplasm; Depth; Development; Devices; Diagnostic; Diagnostic Neoplasm Staging; Dysplasia; Early Diagnosis; Endoscopes; Endoscopy; Excision biopsy; Face; Formalin; Future; Gastroenterology; Gastrointestinal tract structure; Genus Cola; Healthcare Systems; Histology; Image; In Situ; Investigation; Laparoscopes; Libraries; Malignant Neoplasms; Mechanics; Microscope; Microscopy; Mitosis; Morphology; Needles; Nuclear; Optical Biopsy; Optical Coherence Tomography; Optics; Oryctolagus cuniculus; Pathology; Research; Resected; Resolution; Sampling Errors; Scanning; Specimen; Speed; System; Techniques; Technology; Time; Tissue Sample; Tissue Stains; Tissues; Universities; Work; base; cancer cell; cellular imaging; design and construction; human subject; human tissue; imaging probe; improved; in vivo; miniaturize; neoplastic; new technology; research study; technology development

The hypothesis of this proposal is that optical coherence microscopy (OCM), a combination of OCT with confocal microscopy, can enable cellular resolution endoscopic imaging of tissue morphology, thereby dramatically advancing the capabilities of either technique for endoscopic detection of early stage neoplasms. An endoscopic "optical biopsy" technique that allows for real time, in vivo imaging of pre- cancerous changes at the cellular level would allow image guidance of traditional excisional biopsy, potentially reducing sampling error and improving sensitivity. The specific aims are: 1) Develop optical coherence microscopy (OCM) technology for cellular imaging of unstained tissues. 2) Investigate OCM for detecting early neoplastic cellular alterations in human tissues through ex vivo clinical imaging studies. 3) Develop miniaturized flexible endoscopes for OCM imaging based on micro-electro-mechanical systems (MEMS) scanning technology. 4) Validate flexible OCM endoscopes through in vivo animal imaging experiments. These studies combined seek to demonstrate fundamental new advances for endoscopic detection of early stage neoplasms.

该提案的假设是光学相干显微镜 (OCM)，OCT 与 共聚焦显微镜，可以实现组织形态的细胞分辨率内窥镜成像，从而 极大地提高了这两种技术用于早期内窥镜检测的能力 肿瘤。一种内窥镜“光学活检”技术，可以对预活检进行实时体内成像。 细胞水平的癌变将允许传统切除活检的图像引导， 可能减少采样误差并提高灵敏度。具体目标是： 1）开发光学 用于未染色组织细胞成像的相干显微镜 (OCM) 技术。 2) 调查 OCM 通过离体临床成像研究检测人体组织中的早期肿瘤细胞改变。 3） 开发基于微机电系统的用于OCM成像的小型化柔性内窥镜 （MEMS）扫描技术。 4) 通过体内动物成像验证柔性 OCM 内窥镜 实验。这些研究结合起来试图证明内窥镜技术的根本性新进展 早期肿瘤的检测。