Optical Coherence Microscopy for Endoscopic Diagnostics

该建议的假设是，光学相干显微镜（OCM），OCT与 共焦显微镜可以实现组织形态的细胞分辨率内窥镜成像，从而 极大地提高了任一技术用于早期阶段的内窥镜检测的能力， 肿瘤。一种内窥镜“光学活检”技术，允许真实的时间，在体内成像的前， 细胞水平的癌性变化将允许传统切除活检的图像引导， 潜在地减小采样误差并提高灵敏度。具体目标是：1）发展光学 相干显微镜（OCM）技术用于未染色组织的细胞成像。2)调查OCM， 通过离体临床成像研究检测人体组织中的早期肿瘤细胞改变。第三章 基于微机电系统开发用于OCM成像的小型化柔性内窥镜 MEMS扫描技术。4)通过体内动物成像实现柔性OCM内窥镜的可扩展性 实验这些研究旨在证明内窥镜检查的基本新进展 早期肿瘤的检测。

The hypothesis of this proposal is that optical coherence microscopy (OCM), a combination of OCT with confocal microscopy, can enable cellular resolution endoscopic imaging of tissue morphology, thereby dramatically advancing the capabilities of either technique for endoscopic detection of early stage neoplasms. An endoscopic "optical biopsy" technique that allows for real time, in vivo imaging of pre- cancerous changes at the cellular level would allow image guidance of traditional excisional biopsy, potentially reducing sampling error and improving sensitivity. The specific aims are: 1) Develop optical coherence microscopy (OCM) technology for cellular imaging of unstained tissues. 2) Investigate OCM for detecting early neoplastic cellular alterations in human tissues through ex vivo clinical imaging studies. 3) Develop miniaturized flexible endoscopes for OCM imaging based on micro-electro-mechanical systems (MEMS) scanning technology. 4) Validate flexible OCM endoscopes through in vivo animal imaging experiments. These studies combined seek to demonstrate fundamental new advances for endoscopic detection of early stage neoplasms.