Poliovirus Receptor and Poliovirus Pathogenesis

描述(申请人提供)：脊髓灰质炎病毒在摄入和进入胃肠道(Gl)后，可在胃肠道相关淋巴组织(GALT)中复制，随后传播到体循环和中枢神经系统。迄今为止，许多研究转基因小鼠口服脊髓灰质炎病毒感染的尝试都以失败告终。本应用的一个重点是构建一种新型的口服脊髓灰质炎病毒转基因小鼠模型，该模型可用于确定脊髓灰质炎病毒在Gl区的增殖部位(S)。这项应用的第二个重点是研究脊髓灰质炎病毒在极化组织培养细胞、神经元起源的细胞和轴突中迁移的机制(S)。实验基于我们的假设，即脊髓灰质炎病毒受体CD155和Tctex-1之间的相互作用是脊髓灰质炎病毒沿着微管移动的原因。Tctex-1是动力蛋白马达复合体的一种货物结合多肽。

DESCRIPTION (provided by applicant): Following ingestion and entry into the gastrointestinal (Gl) tract, poliovirus, a human neurotropic enterovirus of Picornaviridae, may replicate in gastrointestinal-associated lymphatic tissues (GALT) and, subsequently, spread to the systemic circulation and to the CNS. Numerous attempts to study oral poliovirus infection in transgenic mice have so far failed. A focus of this application is to construct a novel transgenic mouse model for oral poliovirus infection that can be used to determine the site(s) of poliovirus proliferation in the Gl tract. A second focus of this application is to study mechanism(s) by which poliovirus is migrating in polarized tissue culture cells, in cells of neuronal origin, and in axons. Experiments have been developed based on our hypothesis that an interaction between the poliovirus receptor CD155 and Tctex-1, a cargo binding polypeptide of the dynein motor complex, is responsible for movement of poliovirus along microtubules.