UNC Neuroscience Center Research Cores

描述(由申请人提供)： 北卡罗来纳大学教堂山分校的神经科学中心建议建立、安置和配备五个核心设施，以支持神经科学研究的分子、遗传和高分辨率成像方法。拟议的四个核心将增强13名NINDS受赠者的遗传学能力，这些受赠者研究神经发育和神经疾病中的信号传递。它们是：核心1.表达谱和生物信息学，核心2.表达定位，核心3.BAC工程技术，核心4.胚胎干细胞技术。这些核心将提供综合服务，以支持基于分子遗传和突变的方法。核心5.多光子和共焦成像，将与北卡罗来纳大学的神经发育障碍研究中心(NDRC)共同建立和资助。该核心将支持在携带转基因或在核心3和4协助下产生的突变的单细胞、脑片和活动物中迁移、轴突投射和树突生长的可视化。这些核心将被安置在分配给神经科学中心的空间内，位于医学院校园内最近完工的一座8层高的神经科学研究大楼内。北卡罗来纳大学医学院和北卡罗来纳州生物技术中心已承诺投入48万美元建立这些核心设施。这些核心将支持和扩展13个NINDS受资助者的研究工作。所有这些研究人员都是北卡罗来纳大学神经科学中心的成员，在北卡罗来纳大学医学院和系部的基础和临床部门担任联合或初级职务。在主校区的化学系。其中八个受赠者位于毗邻或非常接近的新建实验室空间。在神经系统发育或再生信号的分子方法方面具有专业知识的资深研究人员将监督这些核心。此外，最近招聘的四名年轻调查员是所涉技术的专家，预计在不久的将来将成为国家核数据系统的受赠人，他们将帮助指导核心工作，也将是用户。已经制定了一项谨慎的操作计划，以管理拥有合格项目的13名NINDS受资助者的使用情况；最近招募的四名年轻调查人员，发改委研究大脑发育的一组调查人员，以及北卡罗来纳大学教堂山校区的其他15名NINDS受资助者。这些核心服务的质量和相关性以及它们的有效运作将大大提高NINDS资助的北卡罗来纳大学教堂山分校神经科学研究的生产率。

DESCRIPTION (provided by applicant): The Neuroscience Center at the University of North Carolina-Chapel Hill proposes to establish, house, and staff five Core facilities to support molecular, genetic, and high-resolution imaging approaches for neuroscience research. Four of the proposed Cores will enhance the genetics capabilities of a group of 13 NINDS grantees studying signaling in neural development and neurological disease. They are: Core 1. Expression Profiling and Bioinformatics, Core 2. Expression Localization, Core 3. BAC Engineering Technology, and Core 4. Embryonic Stem Cell Technology. These cores will provide integrated services to support molecular genetic and mutagenesis-based approaches. Core 5. Multiphoton and Confocal Imaging, will be jointly established and funded with the Neurodevelopmental Disorders Research Center (NDRC) at UNC. This core will support visualization of migration, axon projection, and dendritic growth in single cells, brain slices and living animals carrying transgenes or mutations generated with the assistance of Cores 3 and 4. The Cores will be housed within space allotted to the Neuroscience Center in a recently completed 8-story Neuroscience Research Building on the School of Medicine campus. The UNC School of Medicine and the North Carolina Biotechnology Center have committed $480,000 toward establishing these Core facilities. The research efforts of 13 NINDS grantees will be supported and extended by the Cores. All of these investigators are members of the UNC Neuroscience Center with joint or primary appointments in basic and clinical departments at the UNC School of Medicine and in the Dept. of Chemistry on the main campus. Eight of the grantees are in adjacent or closely proximate newly constructed laboratory space. Established investigators with expertise in molecular approaches to signaling in the developing or regenerating nervous system will supervise the Cores. In addition, four recently recruited young investigators who are expert in the technologies involved and who are expected to be NINDS grantees in the near future will help direct the Cores and will also be users. A careful operational plan has been developed to govern usage by the group of 13 NINDS grantees with qualifying projects; the four recently recruited young investigators, a group of investigators in the NDRC studying brain development, and the other 15 NINDS grantees on the UNC-Chapel Hill Campus. The quality and relevance of these Core services and their efficient operation will greatly enhance the productivity of NINDS-funded neuroscience research at UNC-Chapel Hill.