Role of mRNA Decay in the Immune System

mRNA 衰变在免疫系统中的作用

• 批准号: 7382290
• 负责人: SIDNEY PESTKA
• 金额: $ 1.69万
• 依托单位: UNIV OF MED/DENT NJ-R W JOHNSON MED SCH
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-15 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7382290
• 关键词: Role; mRNA; Decay; Immune; System

This Program Project application "The Role of mRNA Decay in the Immune System" is designed to coordinate the efforts of investigators who have been studying post-transcriptional regulation, in particular, the mechanisms involved in mRNA turnover. The program is to be directed by Sidney Pestka, chair of the Department of Molecular Genetics, Microbiology and Immunology and an investigator with a long term interest in interferons, cytokines, signal transduction and mRNA turnover. The Program Project consists of five projects: 1. Adherence Activated Monocyte Genes; 2. ARE & non-ARE Pathways Regulating CD154 mRNA Stability; 3. Translational Regulation by the TNF Alpha AU-rich Element; 4. Regulation of Cytokine Gene Expression by mRNA Turnover; 5: Regulation of RANKL Expression in T-Lymphocytes. Four of the individuals have experience in study of mRNA lifetime; two of the investigators are immunologists. The Program Project contains an administrative core and six small cores to make the work efficient and effective. The cores are: A) Administrative Facility; B) Flow Cytometry Facility; C) Real-Time PCR Facility; C) Imaging Facility; E) Irradiation Facility; F) DNA Synthesis & Sequencing Facility; G) Realtime Fluorescence Resonance Energy Facility. The overall aim of this application is to understand how mRNA turnover controls various steps in immune activation and differentiation during immune responses. One of the fast methods to control changes in protein expression is by modification of the rate ofmRNA decay, a post-transcriptional process. Understanding the role of mRNA turnover in regulation of the immune develop new therapeutics to control these processes and treat a variety and other immune disorders.

这个项目申请“mRNA衰变在免疫系统中的作用”旨在 协调一直在研究转录后调控的研究人员的努力，特别是， 参与mRNA周转的机制。该计划将由悉尼佩斯特卡，主席 分子遗传学，微生物学和免疫学系和一名长期研究员 对干扰素、细胞因子、信号转导和mRNA周转感兴趣。该计划项目包括 五个项目：粘附激活单核细胞基因; 2. ARE和非ARE途径调节CD 154 mRNA稳定性; 3.富含TNF α AU的元件的翻译调节; 4.调控 通过mRNA转换的细胞因子基因表达; 5：T淋巴细胞中RANKL表达的调节。 其中四人有研究mRNA寿命的经验;两名研究人员是 免疫学家该计划项目包含一个行政核心和六个小核心，使工作 高效和有效。核心是：A）管理设施; B）流式细胞仪设施; C）实时 PCR设备; C）成像设备; E）辐照设备; F）DNA合成和测序设备; G） 实时荧光共振能量设施。 本申请的总体目的是了解mRNA周转如何控制免疫调节的各个步骤。 在免疫应答期间激活和分化。一个快速的方法来控制变化， 蛋白质的表达是通过改变mRNA的衰变率，这是一个转录后过程。 了解mRNA周转在免疫调节中的作用 开发新的治疗方法来控制这些过程， 和其他免疫系统疾病。