Simple and Inexpensive Diagnostic Tools for Yeast Infections

简单且廉价的酵母菌感染诊断工具

• 批准号: 7482629
• 负责人: Mitchell C. Sanders
• 金额: $ 20.09万
• 依托单位: ECI BIOTECH
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7482629
• 关键词: Adherence; Affinity; Age; Animals; Antibiotic Therapy; Aspartic Endopeptidases; Bacteria; Bacterial Vaginosis; Binding; Biochemical; Biological Assay; Burn injury; Candida; Candida albicans; Candidiasis; Cell Line; Cell Wall; Cells; Chemistry; Class; Clinical; Clinical Microbiology; Color; Complex; Condition; Depth; Detection; Diabetes Mellitus; Diagnosis; Diagnostic; Disease; Dyes; Endopeptidases; Environment; Enzymes; Event; Failure; Family; Filament; Genital system; Goals; Healthcare Systems; Home environment; Hormones; Hour; Hyphae; Immune system; Infection; Inositol; Lactobacillus; Lateral; Life; Link; Marketing; Mechanics; Membrane; Metalloproteases; Methods; Microbial Biofilms; Microscopy; Mus; NRG1 gene; Necrosis; Neuregulin 1; Nutrient; Outpatients; PLAB Protein; Patients; Penetration; Peptide Hydrolases; Peptides; Phase; Play; Pregnancy; Production; Protein Biochemistry; Proteins; Pruritus; Public Health; Quality of life; Rate; Research; Research Personnel; Role; Sampling; Sensitivity and Specificity; Serum; Sex Behavior; Surface; Swab; Symptoms; System; Technology; Testing; Tetracycline Control; Tissues; Trichomonas Vaginitis; Vagina; Vaginal Discharge; Vaginitis; Virulence; Vulvovaginitis; Woman; Work; Yeasts; assay development; base; commercialization; cost; cytokine; engineering design; fungus; high throughput screening; improved; in vivo; macrophage; microbial; microbial host; mouse model; mutant; pathogen; point of care; promoter; protein aminoacid sequence; prototype; quorum sensing; reproductive; response; self diagnosis; sensor; tool; uptake

DESCRIPTION (provided by applicant): Yeast infections (Candida vaginitis) are a very common worldwide ailment in women of reproductive age. The symptoms of CV include vaginal discharge, itching, and burning; although not life threatening they are certainly not trivial to the patient and can impact the quality of life with a burden on the US healthcare system of $1 billion per year. If there was an inexpensive and easy to use diagnostic for yeast infections, then consumers could avoid the unnecessary therapy (and cost) and women who still have the symptoms but do not have Candida vaginitis could then seek a doctor for more appropriate therapy. Clinicians in an outpatient setting will also benefit from having a rapid test for yeast infections that will reduce improper diagnosis and provide more accurate treatment regiment. The long term goal and objective of this phase I project is to develop a rapid and simple consumer diagnostic for yeast infections. Specifically based on a high throughput screen, we have identified 7 potential targets for a yeast diagnostic that will be tested with clinical vaginal swab samples. Once we have refined and chosen the best diagnostic target using biochemical approaches we will convert this bench assay into a home pregnancy-like rapid test format and then retest it with clinical sample to delineate the sensitivity and specificity of the test. The methods employed in this project include 1)protein biochemistry for conjugating the enzyme target to affinity tags 2)clinical microbiology of genital tract infections and assay development to test the potential diagnostic targets and correlate the activities with disease, 3) micro-fluidics and lateral flow expertise to convert the bench chemistry into a membrane based system and 4) in phase II mechanical design and engineering to incorporate the components into a stand alone point of care rapid (5 minutes) diagnostic. PUBLIC HEALTH RELEVANCE: Yeast infections are a very common ailment. The benefit to the public health is the commercialization of a rapid diagnostic product for yeast infections that will allow women to reliably self-treat or seek a doctor if it is something other than a yeast infection.

描述（申请人提供）：酵母菌感染（念珠菌阴道炎）是一种非常常见的世界性疾病，在育龄妇女。CV的症状包括阴道分泌物、瘙痒和灼烧；虽然不会危及生命，但它们对患者来说肯定不是微不足道的，而且会影响生活质量，每年给美国医疗保健系统带来10亿美元的负担。如果有一种既便宜又容易使用的酵母菌感染诊断方法，那么消费者就可以避免不必要的治疗（和费用），那些仍然有症状但没有念珠菌阴道炎的妇女就可以寻求医生更合适的治疗方法。门诊的临床医生也将受益于对酵母菌感染的快速检测，这将减少不正确的诊断并提供更准确的治疗方案。这个第一阶段项目的长期目标和目的是开发一种快速和简单的酵母菌感染消费者诊断方法。具体来说，基于高通量筛选，我们已经确定了7个潜在的酵母菌诊断靶点，将用临床阴道拭子样本进行测试。一旦我们使用生化方法完善并选择了最佳诊断靶点，我们将把这种实验室试验转换为家庭妊娠样快速测试格式，然后用临床样本重新测试，以确定该测试的敏感性和特异性。本项目采用的方法包括：1)蛋白质生物化学将酶靶点与亲和标签结合；2)生殖道感染的临床微生物学和检测开发，以检测潜在的诊断靶点并将其与疾病的活性联系起来；3)微流体和横向流动专业知识，将实验化学转化为基于膜的系统；4)在第二阶段的机械设计和工程中，将组件整合到独立的护理点快速诊断（5分钟）中。公共卫生相关性：酵母菌感染是一种非常常见的疾病。对公众健康有益的是一种酵母菌感染快速诊断产品的商业化，这将使妇女能够可靠地自我治疗，或者如果不是酵母菌感染就去看医生。