On the Protein Folding Problem

关于蛋白质折叠问题

• 批准号: EP/E014062/1
• 负责人: Anthony Harriman
• 金额: $ 12.87万
• 依托单位: Newcastle University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FE014062%2F1
• 关键词: Protein; Folding; Problem

Proteins are the biological workhorses that carry out vital functions in living cells. Before they can carry out their required functions, the proteins must fold into a complex three-dimensional structure but the underlying factors that perform this folding process remain unclear. Although the steps leading to protein folding are fast and highly efficient, things sometimes go wrong. This can have harmful consequences, as the incorrect folding of proteins is thought to be the cause of many diseases, such as Alzheimer's disease and certain types of cancer. We propose here to build an instrument that will enable the systematic study of the dynamics of protein folding over a very wide temporal range. A key feature of the new instrument will involve a high-pressure cell that allows conformational motion to be controlled by varying frictional forces. The instrument will be used to follow large-scale unfolding of a short protein triggered by breaking a disulphide bond. This study should expose the mechanism and thermodynamics associated with the unfolding and refolding processes. Subsequent work should help unravel the role of metal ions in promoting Alzheimer's disease.

蛋白质是在活细胞中执行重要功能的生物学工具。在它们能够执行其所需的功能之前，蛋白质必须折叠成复杂的三维结构，但执行这种折叠过程的潜在因素仍不清楚。虽然导致蛋白质折叠的步骤是快速和高效的，但有时会出错。这可能会产生有害的后果，因为蛋白质的错误折叠被认为是许多疾病的原因，如阿尔茨海默病和某些类型的癌症。在这里，我们建议建立一个仪器，将使系统的研究在很宽的时间范围内的蛋白质折叠的动力学。新仪器的一个关键特征将涉及一个高压单元，该单元允许通过变化的摩擦力来控制构象运动。该仪器将用于跟踪由断裂二硫键引发的短蛋白质的大规模解折叠。这项研究将揭示与去折叠和重折叠过程相关的机制和热力学。后续的工作应该有助于解开金属离子在促进阿尔茨海默病中的作用。

项目成果

Electronic energy transfer in molecular dyads built around boron-ethyne-substituted subphthalocyanines.

围绕硼乙炔取代的亚酞菁构建的分子二元体中的电子能量转移。

• DOI: 10.1002/chem.200900394
• 发表时间: 2009
• 期刊: Chemistry (Weinheim an der Bergstrasse, Germany)
• 作者: Ziessel R

Through-space electronic energy transfer across proximal molecular dyads.

• DOI: 10.1002/anie.201302081
• 发表时间: 2013-06
• 期刊: Angewandte Chemie
• 作者: A. Harriman;Mohammed A. H. Alamiry;J. Hagon;Delphine Hablot;R. Ziessel

Influence of applied pressure on the probability of electronic energy transfer across a molecular dyad

施加压力对分子二联体电子能量转移概率的影响

• DOI: 10.1351/pac-con-12-09-04
• 发表时间: 2013
• 期刊: Pure and Applied Chemistry
• 影响因子: 1.8
• 作者: Alamiry M

Providing power for miniaturized medical implants: triplet sensitization of semiconductor surfaces.

为微型医疗植入物提供动力：半导体表面的三重态敏化。

• DOI: 10.1098/rsta.2012.0334
• 发表时间: 2013
• 期刊: Philosophical transactions. Series A, Mathematical, physical, and engineering sciences
• 作者: Benniston AC

Solid-state gas sensors developed from functional difluoroboradiazaindacene dyes.

• DOI: 10.1002/chem.200801911
• 发表时间: 2009-01
• 期刊: Chemistry
• 作者: R. Ziessel;G. Ulrich;A. Harriman;Mohammed A. H. Alamiry;B. Stewart;P. Retailleau