Herpes Simplex Virus Capsid Assembly and DNA Packaging

单纯疱疹病毒衣壳组装和 DNA 包装

基本信息

项目摘要

Infections with human herpesviruses are endemic in the population with some herpesviruses causing severe disease, especially in immune impaired individuals and newborns. The increased immunocompromised population has created an unmet medical need for antivirals against herpesviruses. The goal of the proposed project is to gain a better understanding of DNA cleavage and packaging in herpes simplex virus (HSV) through the analysis of two viral proteins, UL25 and UL28, that are required for this process. The specific hypothesis behind the proposed research is that UL25 and UL28 are required for packaging DNA into HSV capsids at separate stages in the cleavage packaging reaction. The UL28 protein is required for cleavage of concatemeric DNA into unit length molecules while UL25 serves an essential function in the production of DNA containing capsids. Although, the focus will be on UL25 and UL28 the goal is to characterize the cleavage/packaging process using genetic and biochemical approaches to examine how mutations in the UL25 and UL28 genes affect the interaction of these proteins with: (i) DNA, (ii) capsid proteins, (iii) cleavage/packaging proteins and (iv)host cell proteins. Specific goals of the project are to: 1. Determine the capsid location, DNA binding specificity and protein-protein interactions of UL25. 2. Identify functional domains of UL28 that are important for capsid incorporation, binding viral DNA packaging sites and for interaction with terminase (UL15) and portal protein (UL6) by characterizing a series of HSV-1 UL28 mutants that we have isolated; and map the second site mutation for a revertent of one of the lethal UL28 linker- insertion mutants since marker rescue and DNA sequencing have demonstrated that the mutation does not map to the UL28 gene. 3. Examine the interaction of UL25 and UL28 with viral and cell proteins using proteomic and biochemical approaches in order to identify essential protein-protein interactions and protein complexes that are involved in the cleavage/packaging reaction. These studies aim to elucidate the mechanism underlying HSV DNA cleavage and packaging and may suggest novel targets for the development of antivirals.
人类疱疹病毒感染在人群中是地方性的,一些疱疹病毒引起严重的 疾病,特别是免疫受损的个体和新生儿。免疫功能低下的增加 人口的增加产生了对针对疱疹病毒的抗病毒药物的未满足的医疗需求。建议的目标 一个项目是为了更好地了解单纯疱疹病毒(HSV)中的DNA切割和包装 通过分析这一过程所需的两种病毒蛋白质UL 25和UL 28。具体 这项研究背后的假设是,UL 25和UL 28是将DNA包装到HSV中所必需的 在裂解包装反应中的不同阶段分离衣壳。UL 28蛋白是切割 UL 25可以将多联体DNA转化为单位长度的分子,而UL 25在产生多联体DNA中起重要作用。 含有DNA的衣壳。虽然重点将放在UL 25和UL 28上,但目标是表征 切割/包装过程,使用遗传和生物化学方法来检查突变是如何在 UL 25和UL 28基因影响这些蛋白质与以下物质的相互作用:(i)DNA,(ii)衣壳蛋白,(iii) 切割/包装蛋白和(iv)宿主细胞蛋白。该项目的具体目标是:1。确定 UL 25的衣壳位置、DNA结合特异性和蛋白质-蛋白质相互作用。2.识别功能 UL 28的结构域对于衣壳掺入、结合病毒DNA包装位点和 通过表征一系列HSV-1 UL 28突变体与末端酶(UL 15)和门蛋白(UL 6)的相互作用 我们已经分离出来了;并绘制第二个位点突变的致命UL 28接头之一的回复体- 插入突变体,因为标记拯救和DNA测序已经证明突变不 与UL 28基因相关。3.检测UL 25和UL 28与病毒和细胞蛋白的相互作用, 蛋白质组学和生物化学方法,以确定必要的蛋白质-蛋白质相互作用和蛋白质 参与切割/包装反应的复合物。这些研究旨在阐明 HSVDNA切割和包装的潜在机制,并可能为HSVDNA切割和包装提供新的靶点。 开发抗病毒药物。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

FREDERICK L HOMA其他文献

FREDERICK L HOMA的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('FREDERICK L HOMA', 18)}}的其他基金

Herpes Simplex Virus Capsid Assembly and DNA Packaging
单纯疱疹病毒衣壳组装和 DNA 包装
  • 批准号:
    7151141
  • 财政年份:
    2005
  • 资助金额:
    $ 31.83万
  • 项目类别:
Herpes Simplex Virus Capsid Assembly and DNA Packaging
单纯疱疹病毒衣壳组装和 DNA 包装
  • 批准号:
    7737371
  • 财政年份:
    2005
  • 资助金额:
    $ 31.83万
  • 项目类别:
Herpes Simplex Virus Capsid Assembly and DNA Packaging
单纯疱疹病毒衣壳组装和 DNA 包装
  • 批准号:
    7037025
  • 财政年份:
    2005
  • 资助金额:
    $ 31.83万
  • 项目类别:
Herpes Simplex Virus Capsid Assembly and DNA Packaging
单纯疱疹病毒衣壳组装和 DNA 包装
  • 批准号:
    7534331
  • 财政年份:
    2005
  • 资助金额:
    $ 31.83万
  • 项目类别:
Herpes Simples Virus Capsid Assembly and DNA Packaging
单纯疱疹病毒衣壳组装和 DNA 包装
  • 批准号:
    8337100
  • 财政年份:
    2004
  • 资助金额:
    $ 31.83万
  • 项目类别:

相似海外基金

Cellular membrane affinity chromatography kit for drug discovery
用于药物发现的细胞膜亲和层析试剂盒
  • 批准号:
    10506915
  • 财政年份:
    2021
  • 资助金额:
    $ 31.83万
  • 项目类别:
Cellular membrane affinity chromatography kit for drug discovery
用于药物发现的细胞膜亲和层析试剂盒
  • 批准号:
    10325006
  • 财政年份:
    2021
  • 资助金额:
    $ 31.83万
  • 项目类别:
SBIR Phase I: A New Class of Immobilized Metal Affinity Chromatography Resins
SBIR 第一阶段:一类新型固定金属亲和色谱树脂
  • 批准号:
    1746198
  • 财政年份:
    2018
  • 资助金额:
    $ 31.83万
  • 项目类别:
    Standard Grant
Marine speciation of nickel using immobilized nickel affinity chromatography
使用固定镍亲和色谱法测定镍的海洋形态
  • 批准号:
    512537-2017
  • 财政年份:
    2017
  • 资助金额:
    $ 31.83万
  • 项目类别:
    University Undergraduate Student Research Awards
I-Corps: Commercialization of Immobilized Metal Affinity Chromatography Resins Based on Nanomaterials
I-Corps:基于纳米材料的固定化金属亲和层析树脂的商业化
  • 批准号:
    1404605
  • 财政年份:
    2014
  • 资助金额:
    $ 31.83万
  • 项目类别:
    Standard Grant
Antibody Purification via Affinity Chromatography that Utilizes the Unconventional Nucleotide Binding Site
利用非常规核苷酸结合位点通过亲和色谱法纯化抗体
  • 批准号:
    1263713
  • 财政年份:
    2013
  • 资助金额:
    $ 31.83万
  • 项目类别:
    Continuing Grant
Development of multivalent DNA network based affinity chromatography diagnostics for isolating circulating tumour cells
开发基于多价 DNA 网络的亲和色谱诊断法,用于分离循环肿瘤细胞
  • 批准号:
    425749-2012
  • 财政年份:
    2012
  • 资助金额:
    $ 31.83万
  • 项目类别:
    Postgraduate Scholarships - Master's
Next-Generation Affinity Chromatography with PEGylated Ligands
使用聚乙二醇化配体的新一代亲和色谱法
  • 批准号:
    1159886
  • 财政年份:
    2012
  • 资助金额:
    $ 31.83万
  • 项目类别:
    Standard Grant
Immobilized zirconium ion affinity chromatography for specific enrichment of phosphoproteins
用于磷蛋白特异性富集的固定化锆离子亲和层析
  • 批准号:
    19560760
  • 财政年份:
    2007
  • 资助金额:
    $ 31.83万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Accelerating drug discovery using frontal affinity chromatography/mass spectrometry
使用正面亲和色谱/质谱加速药物发现
  • 批准号:
    234753-2000
  • 财政年份:
    2003
  • 资助金额:
    $ 31.83万
  • 项目类别:
    Collaborative Research and Development Grants
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了