CORE--Genomics-Proteomics
核心--基因组学-蛋白质组学
基本信息
- 批准号:7673356
- 负责人:
- 金额:$ 28.43万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:
- 资助国家:美国
- 起止时间:至
- 项目状态:未结题
- 来源:
- 关键词:2&apos-deoxycytidine 5&apos-triphosphateADP ribosylationAccountingAcetonitrilesAcetylationAcidsAcrylamideAcrylamidesActinsAddressAffinityAffinity ChromatographyAgar Gel ElectrophoresisAgeAlbuminsAldehydesAliquotAm 80Amino Acid SequenceAmino AcidsAmpholytesAntibodiesAntibody FormationAntisense RNAArtsBacteriaBindingBinding ProteinsBioinformaticsBiologicalBiological AssayBiometryBiotechnologyBiotinBlast CellBlood capillariesBuffersCalciumCaliberCalibrationCapillary ElectrophoresisCardiacCardiac MyocytesCardiovascular systemCaseinsCell FractionCell FractionationCell NucleusCell membraneCell physiologyCellsCentrifugationCharacteristicsChargeChimeric ProteinsChloroformChromatographyClassClassificationCleaved cellCloningCluster AnalysisCollaborationsCompatibleComplementary DNAComplexComputer softwareConditionCoomassie blueCore FacilityCoupledCustomCyclophilinsCytolysisCytosolD CellsDNADNA Microarray ChipDNA Polymerase IDNA SequenceDataData AnalysesData CollectionDatabasesDensitometryDepthDetectionDevicesDigestionDimensionsDisadvantagedDiseaseDoctor of PhilosophyDouble-Stranded RNADyesEdetic AcidElectrophoresisEndopeptidasesEnzyme-Linked Immunosorbent AssayEpitopesEquipmentEvaluationExcisionExpressed Sequence TagsFamily suidaeFluoresceinFluoresceinsFluorescenceFluorescent DyesFluorescent ProbesFormaldehydeFractionationFreezingFundingGalliumGasesGelGel ChromatographyGene ExpressionGene Expression RegulationGene ProteinsGeneric DrugsGenesGeneticGenomeGenomicsGenus LynxGlutathione S-TransferaseGlycerolHarvestHeartHeatingHigh Pressure Liquid ChromatographyHourHouse miceHousekeepingHousekeeping GeneHousingHydration statusHydrophobic InteractionsImageImage AnalysisIn SituIn Situ HybridizationIn VitroIncubatedIncubatorsIndividualIodoacetamideIon ExchangeIonsIsoelectric FocusingIsoelectric PointIsotopically-Coded Affinity TaggingKnock-outLabelLaboratoriesLaemmli bufferLasersLengthLibrariesLifeLigationLinkLiquid ChromatographyLiquid substanceMALDI-TOF Mass SpectrometryMaintenanceMapsMass ChromatographyMass Spectrum AnalysisMeasurementMeasuresMediatingMembraneMessenger RNAMetalsMethodsMicroarray AnalysisMitochondriaModelingModificationMolecularMolecular CloningMolecular ProbesMolecular WeightMonitorMonkeysMusNitrogenNucleic AcidsNumbersNutsNylonsOligonucleotidesOnline SystemsOrangesParaffinParentsPathologyPatternPeptide FragmentsPeptide HydrolasesPeptide antibodiesPeptidesPeptidylprolyl IsomerasePhasePhenolsPhosphopeptidesPhosphoproteinsPhosphoric Monoester HydrolasesPhosphorylated PeptidePhosphorylationPhosphorylation SitePhosphotyrosinePhysiologyPlayPoly(A)+ RNAPolyacrylamide Gel ElectrophoresisPolymerasePolymerase Chain ReactionPolymersPost-Translational Protein ProcessingPreparationPrincipal InvestigatorPrintingProbabilityProceduresProcessPromegaProtein AnalysisProtein Array AnalysisProtein DatabasesProtein OverexpressionProteinsProteomeProteomicsProtocols documentationPublishingPurposePusPyroxylinRNARNA ProcessingRNA purificationRNA-Directed DNA PolymeraseRangeReactionReadingReagentRegulationRelative (related person)ReportingReproducibilityResearchResearch PersonnelResolutionReverse Transcriptase Polymerase Chain ReactionRibosomal ProteinsRobotRoboticsRoleRunningSamplingScanningScreening procedureSepharoseSequence AnalysisSerineServicesSignal TransductionSignal Transduction PathwaySilverSilver StainingSiteSodium Dodecyl Sulfate-PAGESolutionsSorting - Cell MovementSourceSpecificitySpectrometrySpectrometry, Mass, Electrospray IonizationSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationSpeedSportsSpottingsStaining methodStainsStandards of Weights and MeasuresStructureSucroseSus scrofaSwissProtSystemTechniquesTechnologyTestingThiocyanatesThioureaThrombinTimeTissue SampleTissuesTranscriptTransgenic MiceTransgenic OrganismsTranslationsTransmembrane DomainTrifluoroacetic AcidTriton X100TrypsinTwo-Dimensional Gel ElectrophoresisUnited States National Institutes of HealthUreaValidationVariantVendorWaterWestern BlottingWorkYeastsamino groupammonium bicarbonateaustinbasecDNA Librarycapillarycellular targetingcinnamic acidcomparativecostcross reactivitycyanine dye 5data acquisitiondaydesigndetectordimerfluorophoreformamidegel electrophoresisgene cloninggenome sequencingglycosylationhuman TYRP1 proteinimmunocytochemistryimprovedin vivoinhibitor/antagonistinsightinstrumentinterestmass spectrometermicrobial alkaline proteinase inhibitormolecular dynamicsmouse genomemouse modelnanonovelnucleic acid quantitationoxidationpH gradientphosphatase inhibitorpolyclonal antibodypolyvinylidene fluorideprogramspromoterprotein aminoacid sequenceprotein expressionprotein functionprotein protein interactionresearch studyreversed phase chromatographysample collectionsmall moleculesodium citratestatisticssynthetic peptidetandem mass spectrometrythiocyanatetwo-dimensionalvectoryeast two hybrid system
项目摘要
The Genomics/Proteomics Core is designed to support all the Projects in the Program Project for the
Specific Aims that address the regulation of gene and protein expression. The Genomics/ Proteomics Core
is also designed to interact with all the other Cores, especially Core-D (Biostatistics/Bioinformatics).
Genomics and Proteomics are combined to offer a link, both logical and biological, between gene
expression, transcript stability, protein translation and post-translational modifications. The Core will
combine focused techniques (to investigate one specific product of interest) and large-scale profiling at both
the gene and protein levels. The Genomics/ Proteomics Core will provide technical assistance to the
different Projects for the processing of samples, whereas the preparation of the samples themselves will be
the responsibility of the investigators from the different Projects and from Core B (Physiology). Once the
samples are processed, the raw data will be transmitted to these investigators and to Core D (Biostatistics)
who will be in charge of the data analysis and extraction of results. Additional techniques related to gene
and protein expression that would require fixed samples will be done in collaboration with Core E
(Pathology).
基因组学/蛋白质组学核心旨在支持计划项目中的所有项目,
针对基因和蛋白质表达调控的特定目的。基因组学/蛋白质组学核心
它还被设计为与所有其他核心进行交互,特别是Core-D(生物统计学/生物信息学)。
基因组学和蛋白质组学相结合,提供了一个链接,无论是逻辑和生物,基因之间,
表达、转录物稳定性、蛋白质翻译和翻译后修饰。芯会
联合收割机将重点技术(调查一种特定的感兴趣产品)和大规模分析结合起来,
基因和蛋白质水平。基因组学/蛋白质组学核心将为
不同项目的样品处理,而样品本身的制备将是
不同项目和核心B(生理学)研究者的职责。一旦
处理样本后,将原始数据传输给这些研究者和核心D(生物统计学)
负责数据分析和结果提取。与基因相关的其他技术
需要固定样品的蛋白质表达将与核心E合作完成
(病理学)。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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