CORE--Genomics-Proteomics

核心--基因组学-蛋白质组学

• 批准号: 8133518
• 负责人: Christophe Depre
• 金额: $ 30.79万
• 依托单位: UNIV OF MED/DENT OF NJ-NJ MEDICAL SCHOOL
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8133518
• 关键词: Address; Bioinformatics; Biological; Biometry; Cardiac; Cell Nucleus; Charge; Collaborations; Complementary DNA; Cytosol; Data; Data Analyses; Detection; Gene Expression; Gene Proteins; Genomics; Harvest; In Situ Hybridization; Knock-out; Link; Mass Spectrum Analysis; Methods; Microarray Analysis; Modeling; Modification; Mus; Northern Blotting; Nucleic Acids; Pathology; Phosphorylation; Physiology; Post-Translational Protein Processing; Preparation; Process; Proteins; Proteome; Proteomics; Regulation; Research Personnel; Sampling; Services; Structure; Techniques; Transcript; Transgenic Mice; Translations; Two-Dimensional Gel Electrophoresis; Validation; Western Blotting; cost; design; immunocytochemistry; interest; overexpression; preconditioning; programs; protein expression

The Genomics/Proteomics Core is designed to support all the Projects in the Program Project for the Specific Aims that address the regulation of gene and protein expression. The Genomics/ Proteomics Core is also designed to interact with all the other Cores, especially Core-D (Biostatistics/Bioinformatics). Genomics and Proteomics are combined to offer a link, both logical and biological, between gene expression, transcript stability, protein translation and post-translational modifications. The Core will combine focused techniques (to investigate one specific product of interest) and large-scale profiling at both the gene and protein levels. The Genomics/ Proteomics Core will provide technical assistance to the different Projects for the processing of samples, whereas the preparation of the samples themselves will be the responsibility of the investigators from the different Projects and from Core B (Physiology). Once the samples are processed, the raw data will be transmitted to these investigators and to Core D (Biostatistics) who will be in charge of the data analysis and extraction of results. Additional techniques related to gene and protein expression that would require fixed samples will be done in collaboration with Core E (Pathology).

基因组学/蛋白质组学核心旨在支持计划项目中的所有项目， 针对基因和蛋白质表达调控的特定目的。基因组学/蛋白质组学核心 它还被设计为与所有其他核心进行交互，特别是Core-D（生物统计学/生物信息学）。 基因组学和蛋白质组学相结合，提供了一个链接，无论是逻辑和生物，基因之间， 表达、转录物稳定性、蛋白质翻译和翻译后修饰。芯会 联合收割机将重点技术（调查一种特定的感兴趣产品）和大规模分析结合起来， 基因和蛋白质水平。基因组学/蛋白质组学核心将为 不同项目的样品处理，而样品本身的制备将是 不同项目和核心B（生理学）研究者的职责。一旦 处理样本后，将原始数据传输给这些研究者和核心D（生物统计学） 负责数据分析和结果提取。与基因相关的其他技术 需要固定样品的蛋白质表达将与核心E合作完成 （病理学）。